IL-1 beta
HUABIO
catalog: HA601002

mouse monoclonal (A7F9)
reactivity: human, mouse, rat
application: western blot, ELISA

Western blot analysis of IL-1 beta on different lysates with Mouse anti-IL-1 beta antibody (HA601002) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31/28/20 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601002) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-1 beta on different lysates with Mouse anti-IL-1 beta antibody (HA601002) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 minutes 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601002) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-1 beta on different proteins with Mouse anti-IL-1 beta antibody (HA601002) at 1/2,000 dilution. Lane 1: Recombinant mouse IL-1 beta Lane 2: Recombinant rat IL-1 beta Lane 3: Recombinant human IL-1 beta Lysates/proteins at 50 ng/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601002) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

IL-1 beta
HUABIO
catalog: HA601036

mouse monoclonal (A7F7-R)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry - paraffin section

Western blot analysis of IL-1 beta on different lysates with Mouse anti-IL-1 beta antibody (HA601036) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601036) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-1 beta on different proteins with Mouse anti-IL-1 beta antibody (HA601036) at 1/2,000 dilution. Lane 1: Recombinant mouse IL-1 beta Lane 2: Recombinant rat IL-1 beta Lane 3: Recombinant human IL-1 beta Lysates/proteins at 50 ng/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601036) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta antibody (HA601036) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601036) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

IL-1 beta
HUABIO
catalog: HA610004

mouse monoclonal (A7F7-R)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry - paraffin section

Western blot analysis of IL-1 beta on different lysates with Mouse anti-IL-1 beta antibody (HA610004) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-1 beta on different proteins with Mouse anti-IL-1 beta antibody (HA610004) at 1/2,000 dilution. Lane 1: Recombinant mouse IL-1 beta Lane 2: Recombinant rat IL-1 beta Lane 3: Recombinant human IL-1 beta Lysates/proteins at 50 ng/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610004) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta antibody (HA610004) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610004) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

Mouse IL-1 beta
HUABIO
catalog: HA721976

domestic rabbit monoclonal (PSH03-31)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse IL-1 beta matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721976) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse IL-1 beta protein (HA210706) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721977)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of IL-1β were interpolated from the IL-1β standard curve and corrected for sample dilution. The mean IL-1β concentration were determined to be 27pg/mL and 1,615 pg/mL in unstimuated and LPS stimulated Raw264.7 cell extract.
quantity: 100μg
price: 800.00 USD
to the supplier

Mouse IL-1 beta
HUABIO
catalog: HA721977

domestic rabbit monoclonal (PSH03-32)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse IL-1 beta matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721976) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse IL-1 beta protein (HA210706) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721977)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of IL-1β were interpolated from the IL-1β standard curve and corrected for sample dilution. The mean IL-1β concentration were determined to be 27pg/mL and 1,615 pg/mL in unstimuated and LPS stimulated Raw264.7 cell extract.
quantity: 100μg
price: 800.00 USD
to the supplier

Mouse IL-1 beta
HUABIO
catalog: HA722166

domestic rabbit monoclonal (PSH04-66)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-1β matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722166) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1β protein (HA210706) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722167, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-1 beta
HUABIO
catalog: HA722167

domestic rabbit monoclonal (PSH04-67)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-1β matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722166) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1β protein (HA210706) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722167, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-1 beta
HUABIO
catalog: HA722608B

domestic rabbit monoclonal (PSH04-67)
reactivity: mouse
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-1 beta
HUABIO
catalog: HA722625B

domestic rabbit monoclonal (PSH03-32)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of mouse IL-1 beta matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721976) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse IL-1 beta protein (HA210706) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721977)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of IL-1β were interpolated from the IL-1β standard curve and corrected for sample dilution. The mean IL-1β concentration were determined to be 27pg/mL and 1,615 pg/mL in unstimuated and LPS stimulated Raw264.7 cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-1 beta
HUABIO
catalog: HA723120

domestic rabbit monoclonal (PSH09-58)
reactivity: mouse
application: ELISA

Indirect ELISA analysis of Mouse IL-1 beta was performed by coating wells of a 96-well plate with 50 µL per well of Mouse IL-1 beta-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of Mouse IL-1 beta monoclonal antibody serial diluted starting from a concentration of 1µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-1 beta
HUABIO
catalog: HA723121

domestic rabbit monoclonal (PSH09-59)
reactivity: mouse
application: ELISA

Indirect ELISA analysis of Mouse IL-1 beta was performed by coating wells of a 96-well plate with 50 µL per well of Mouse IL-1 beta-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of Mouse IL-1 beta monoclonal antibody serial diluted starting from a concentration of 1µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

IL-1 beta
HUABIO
catalog: HA723965

domestic rabbit monoclonal (PSH18-00)
reactivity: mouse, rat
application: western blot

Western blot analysis of IL-1 beta on different lysates with Rabbit anti-IL-1 beta antibody (HA723965) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate Lane 3: NR8383 cell lysate Lane 4: NR8383 treated with 100ng/mL LPS for 4 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 31 kDa Observed band size: 35/20 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723965) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier