IL-1 alpha
HUABIO
catalog: ER1802-36
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of IL1 alpha on human lymph node lysates using anti-IL1 alpha antibody at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL1 alpha antibody. Counter stained with hematoxylin.

Flow cytometric analysis of PC-3M cells with IL1 alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
quantity: 100μl
price: 330 USD
to the supplier
Mouse IL-1 alpha
HUABIO
catalog: HA723526
domestic rabbit monoclonal (PSH13-40)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723526) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (HA723527, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-1alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours. Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,546 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant.

Interpolated concentrations of spiked IL-1 alpha in cell culture media samples. Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse IL-1 alpha
HUABIO
catalog: HA723527
domestic rabbit monoclonal (PSH13-41)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723526) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (HA723527, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-1 alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours. Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,546 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant.

Interpolated concentrations of spiked IL-1 alpha in cell culture media samples. Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse IL-1 alpha
HUABIO
catalog: HA723528B
domestic rabbit monoclonal (PSH13-41)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723526) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (HA723527, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-1 alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours. Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,546 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant.

Interpolated concentrations of spiked IL-1 alpha in cell culture media samples. Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-41] The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Mouse IL-1 alpha
HUABIO
catalog: HA723529
domestic rabbit monoclonal (PSH13-42)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723529) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (HA723530, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-1 alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours. Capture: HA723529, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723530, Mouse IL-1 alpha Rabbit mAb [PSH13-43] Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,185 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant.

Interpolated concentrations of spiked IL-1 alpha in cell culture media samples. Capture: HA723529, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723530, Mouse IL-1 alpha Rabbit mAb [PSH13-43] The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse IL-1 alpha
HUABIO
catalog: HA723530
domestic rabbit monoclonal (PSH13-43)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723529) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (HA723530, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-1 alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours. Capture: HA723529, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723530, Mouse IL-1 alpha Rabbit mAb [PSH13-43] Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,185 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant.

Interpolated concentrations of spiked IL-1 alpha in cell culture media samples. Capture: HA723529, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723530, Mouse IL-1 alpha Rabbit mAb [PSH13-43] The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse IL-1 alpha
HUABIO
catalog: HA723531B
domestic rabbit monoclonal (PSH13-43)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies Capture: HA723526, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723527, Mouse IL-1 alpha Rabbit mAb [PSH13-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723529) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1,200 pg/ml to 0 pg/ml and detect antibody (HA723530, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-1 alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours. Capture: HA723529, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723530, Mouse IL-1 alpha Rabbit mAb [PSH13-43] Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 1,185 pg/mL in neat Raw264.7 treated supernatant, undetectable in untreated Raw264.7 supernatant.

Interpolated concentrations of spiked IL-1 alpha in cell culture media samples. Capture: HA723529, Mouse IL-1 alpha Rabbit mAb [PSH13-42] Detector: HA723530, Mouse IL-1 alpha Rabbit mAb [PSH13-43] The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
IL-1 alpha
HUABIO
catalog: HA723772
domestic rabbit monoclonal (PSH15-65)
reactivity: mouse
application: western blot

Western blot analysis of IL-1 alpha on different lysates with Rabbit anti-IL-1 alpha antibody (HA723772) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723772) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
IL-1 alpha
HUABIO
catalog: HA723773
domestic rabbit monoclonal (PSH15-66)
reactivity: mouse
application: flow cytometry

Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 10μg/mL LPS for 8 hours labeling IL-1 alpha with Rabbit anti-IL-1 alpha antibody (HA723773) at 1/3,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 alpha antibody (HA723773) at 1/3,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of RAW264.7 cells untreated (left) / treated with 10μg/mL LPS for 8 hours (right) labeling IL-1 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723773, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
IL-1 alpha
HUABIO
catalog: HA751576
domestic rabbit monoclonal (PSH15-65)
reactivity: mouse
application: western blot

Western blot analysis of IL-1 alpha on different lysates with Rabbit anti-IL-1 alpha antibody (HA751576) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751576) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
IL-1 alpha
HUABIO
catalog: HA751577
domestic rabbit monoclonal (PSH15-66)
reactivity: mouse
application: flow cytometry

Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 10μg/mL LPS for 8 hours labeling IL-1 alpha with Rabbit anti-IL-1 alpha antibody (HA751577) at 1/3,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-1 alpha antibody (HA751577) at 1/3,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of RAW264.7 cells untreated (left) / treated with 10μg/mL LPS for 8 hours (right) labeling IL-1 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751577, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier