IL-17A
HUABIO
catalog: ER1902-37

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of IL-17A on recombinant protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/20,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-17A on Rat stomach tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-17A on Human skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

Mouse IL-17A
HUABIO
catalog: HA721606

domestic rabbit monoclonal (PS01-91)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA721606, Mouse IL-17A Rabbit mAb [PS01-91] Detector: HA721607, Mouse IL-17A Rabbit mAb [PS01-92] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721606) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17A protein (HA210791) protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721607, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-17A
HUABIO
catalog: HA721607

domestic rabbit monoclonal (PS01-92)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA721606, Mouse IL-17A Rabbit mAb [PS01-91] Detector: HA721607, Mouse IL-17A Rabbit mAb [PS01-92] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721606) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17A protein (HA210791) protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721607, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-17A
HUABIO
catalog: HA721608

domestic rabbit monoclonal (PS01-92)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA721606, Mouse IL-17A Rabbit mAb [PS01-91] Detector: HA721607, Mouse IL-17A Rabbit mAb [PS01-92] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721606) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-17A protein (HA210791) protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721607, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-17A
HUABIO
catalog: HA722264

domestic rabbit monoclonal (PSH05-35)
reactivity: mouse, rat
application: ELISA

Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722264) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein (HA210791) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA722265, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Mouse lung were stimulated with 5 µg/mL LPS and incubated for 6 days. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution.Undiluted samples are LPS stimulated mouse lung supernatants 50% and unstimulated mouse lung supernatants 50%. The mean IL17A concentration was determined to be 621 pg/mL in LPS stimulated mouse lung supernatant and undetectable in the unstimulated mouse lung supernatant.

Interpolated concentrations of native IL17A in unstimulated or stimulated rat spleen culture supernatant. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Rat spleen were stimulated with 5 µg/mL Concanavalin A and incubated for 2 days.The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution. Undiluted samples are Concanavalin A stimulated rat spleen supernatants 20% and unstimulated rat spleen supernatants 50%. The mean IL17A concentration was determined to be 3,703 pg/mL in Concanavalin A stimulated rat spleen supernatant and undetectable in the unstimulated rat spleen supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-17A
HUABIO
catalog: HA722265

domestic rabbit monoclonal (PSH05-36)
reactivity: mouse, rat
application: ELISA

Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722264) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein (HA210791) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA722265, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Mouse lung were stimulated with 5 µg/mL LPS and incubated for 6 days. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution.Undiluted samples are LPS stimulated mouse lung supernatants 50% and unstimulated mouse lung supernatants 50%. The mean IL17A concentration was determined to be 621 pg/mL in LPS stimulated mouse lung supernatant and undetectable in the unstimulated mouse lung supernatant.

Interpolated concentrations of native IL17A in unstimulated or stimulated rat spleen culture supernatant. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Rat spleen were stimulated with 5 µg/mL Concanavalin A and incubated for 2 days.The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution. Undiluted samples are Concanavalin A stimulated rat spleen supernatants 20% and unstimulated rat spleen supernatants 50%. The mean IL17A concentration was determined to be 3,703 pg/mL in Concanavalin A stimulated rat spleen supernatant and undetectable in the unstimulated rat spleen supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-17A
HUABIO
catalog: HA722600B

domestic rabbit monoclonal (PSH05-36)
reactivity: mouse, rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722264) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein (HA210791) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA722265, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Mouse lung were stimulated with 5 µg/mL LPS and incubated for 6 days. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution.Undiluted samples are LPS stimulated mouse lung supernatants 50% and unstimulated mouse lung supernatants 50%. The mean IL17A concentration was determined to be 621 pg/mL in LPS stimulated mouse lung supernatant and undetectable in the unstimulated mouse lung supernatant.

Interpolated concentrations of native IL17A in unstimulated or stimulated rat spleen culture supernatant. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Rat spleen were stimulated with 5 µg/mL Concanavalin A and incubated for 2 days.The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution. Undiluted samples are Concanavalin A stimulated rat spleen supernatants 20% and unstimulated rat spleen supernatants 50%. The mean IL17A concentration was determined to be 3,703 pg/mL in Concanavalin A stimulated rat spleen supernatant and undetectable in the unstimulated rat spleen supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

IL-17A
HUABIO
catalog: IRS056

domestic rabbit polyclonal
reactivity: mouse

mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with IL-17A (IRS056), CD4 (IRS051) and CD8 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IL-17A antibody (IRS056) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of mouse lung disease model tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IL-17A antibody (IRS056) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 330 USD
to the supplier