domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of IL12B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1803-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysates Lane 2: Mouse placenta tissue lysates
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH04-87)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse IL-12p40 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722193) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant mouse IL-12p40 protein (HA210890) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA722194, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-12 p40 in cell culture supernatant and mouse serum samples. IL-12 p40 was measured in 100% cell supernatant. The concentrations of IL-12 p40 were interpolated from the IL-12 p40 standard curves. The mean IL-12 p40 concentration was determined to be 4055 pg/mL in mouse serum. There was no detectable signal in NIH/3T3 cell supernatant.
Interpolated concentrations of spiked IL-12 p40 in mouse cell culture media samples. The concentrations of IL-12 p40 were measured in duplicates, interpolated from the IL-12 p40 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH04-88)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse IL-12p40 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722193) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant mouse IL-12p40 protein (HA210890) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA722194, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-12 p40 in cell culture supernatant and mouse serum samples. IL-12 p40 was measured in 100% cell supernatant. The concentrations of IL-12 p40 were interpolated from the IL-12 p40 standard curves. The mean IL-12 p40 concentration was determined to be 4055 pg/mL in mouse serum. There was no detectable signal in NIH/3T3 cell supernatant.
Interpolated concentrations of spiked IL-12 p40 in mouse cell culture media samples. The concentrations of IL-12 p40 were measured in duplicates, interpolated from the IL-12 p40 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH04-88)
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA