domestic rabbit monoclonal (PSH12-42)
reactivity: mouse, rat
application: ELISA
reactivity: mouse, rat
application: ELISA
Sandwich ELISA analysis of Mouse IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse/Rat IGF1 protein (HA210936) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Rat IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse/Rat IGF1 protein (HA211009) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Mouse IGF1 in mouse samples. Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Interpolated concentration of native IGF1 was measured in duplicate at different sample concentrations. Undiluted samples were 1.25% mouse serum. The mean IGF1 concentration was determined to be 1,584,637 pg/mL in mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-43)
reactivity: mouse, rat
application: ELISA
reactivity: mouse, rat
application: ELISA
Sandwich ELISA analysis of Mouse IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse/Rat IGF1 protein (HA210936) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Rat IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse/Rat IGF1 protein (HA211009) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Mouse IGF1 in mouse samples. Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Interpolated concentration of native IGF1 was measured in duplicate at different sample concentrations. Undiluted samples were 1.25% mouse serum. The mean IGF1 concentration was determined to be 1,584,637 pg/mL in mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-43)
reactivity: mouse, rat
conjugate: biotin
application: ELISA
reactivity: mouse, rat
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse/Rat IGF1 protein (HA210936) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Rat IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse/Rat IGF1 protein (HA211009) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA725038, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Mouse IGF1 in mouse samples. Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Interpolated concentration of native IGF1 was measured in duplicate at different sample concentrations. Undiluted samples were 1.25% mouse serum. The mean IGF1 concentration was determined to be 1,584,637 pg/mL in mouse serum.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-43)
reactivity: mouse, rat
conjugate: HRP
application: ELISA
reactivity: mouse, rat
conjugate: HRP
application: ELISA
Sandwich ELISA analysis of Rat IGF1 matched pair antibodies Capture: HA725037, Mouse/Rat IGF1 Rabbit mAb [PSH12-42] Detector: HA725038, Mouse/Rat IGF1 Rabbit mAb [PSH12-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725037) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat IGF1 protein (HA211009) starting from 333,000 pg/ml to 0 pg/ml and detect antibody (HA725038, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier