domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.
Immunocytochemistry analysis of PC-3M cells labeling Interferon alpha with Rabbit anti-Interferon alpha antibody (ER1802-4) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Interferon alpha antibody (ER1802-4) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
ICC staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH14-75)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse IFNA matched pair antibodies Capture: HA723669, Mouse Interferon alpha 1 Rabbit mAb [PSH14-75] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723669) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Interferon alpha 1 protein (HA210987) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723670, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of spiked IFN-alpha in mouse cell culture media samples. Capture: HA723669, Mouse Interferon alpha 1 Rabbit mAb [PSH14-75] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] The concentrations of IFN-alpha were measured in duplicates, interpolated from the IFN-alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-76)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse IFNA matched pair antibodies Capture: HA723669, Mouse Interferon alpha 1 Rabbit mAb [PSH14-75] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723669) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Interferon alpha 1 protein (HA210987) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723670, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Mouse IFNA matched pair antibodies Capture: HA723672, Mouse Interferon alpha 1 Rabbit mAb [PSH14-77] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723669) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Interferon alpha 1 protein (HA210987) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723672, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of spiked IFN-alpha in mouse cell culture media samples. Capture: HA723669, Mouse Interferon alpha 1 Rabbit mAb [PSH14-75] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] The concentrations of IFN-alpha were measured in duplicates, interpolated from the IFN-alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-76)
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse IFNA matched pair antibodies Capture: HA723669, Mouse Interferon alpha 1 Rabbit mAb [PSH14-75] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723669) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Interferon alpha 1 protein (HA210987) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723670, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Mouse IFNA matched pair antibodies Capture: HA723672, Mouse Interferon alpha 1 Rabbit mAb [PSH14-77] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723669) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Interferon alpha 1 protein (HA210987) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723672, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of spiked IFN-alpha in mouse cell culture media samples. Capture: HA723669, Mouse Interferon alpha 1 Rabbit mAb [PSH14-75] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] The concentrations of IFN-alpha were measured in duplicates, interpolated from the IFN-alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-77)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse IFNA matched pair antibodies Capture: HA723672, Mouse Interferon alpha 1 Rabbit mAb [PSH14-77] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723669) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Interferon alpha 1 protein (HA210987) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723672, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of spiked IFN-alpha in mouse cell culture media samples. Capture: HA723672, Mouse Interferon alpha 1 Rabbit mAb [PSH14-77] Detector: HA723670, Mouse Interferon alpha 1 Rabbit mAb [PSH14-76] The concentrations of IFN-alpha were measured in duplicates, interpolated from the IFN-alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier