HUABIO beta-catenin antibody

Beta Catenin
HUABIO
catalog: 0407-16

domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot

Western blot analysis of Beta Catenin on different lysates with Rabbit anti-Beta Catenin antibody (0407-16) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0407-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Beta Catenin on mouse liver tissue lysates with Rabbit anti-Beta Catenin antibody (0407-16) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0407-16) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

Western blot analysis of beta Catenin on hybrid fish (crucian-carp) heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0407-16, 1/500) was used in 5% NFDM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 45 mins at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

Beta Catenin
HUABIO
catalog: EM0306

mouse monoclonal (A6-F8)
reactivity: human, mouse, rat
application: western blot, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Beta Catenin on different lysates with Mouse anti-Beta Catenin antibody (EM0306) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Human brain tissue lysate (40 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HT-29 cells labeling Beta Catenin with Mouse anti-Beta Catenin antibody (EM0306) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta Catenin antibody (EM0306) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Beta Catenin antibody (EM0306) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0306) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

Beta Catenin
HUABIO
catalog: ER0805

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Beta Catenin on different lysates with Rabbit anti-Beta Catenin antibody (ER0805) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Human brain tissue lysate (40 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HT-29 cells labeling Beta Catenin with Rabbit anti-Beta Catenin antibody (ER0805) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beta Catenin antibody (ER0805) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of C6 cells labeling Beta Catenin with Rabbit anti-Beta Catenin antibody (ER0805) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beta Catenin antibody (ER0805) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier

Beta Catenin
HUABIO
catalog: ET1601-5

domestic rabbit monoclonal (SA30-04)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Beta Catenin on different lysates with Rabbit anti-Beta Catenin antibody (ET1601-5) at 1/2,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-5) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Beta Catenin on different lysates with Rabbit anti-Beta Catenin antibody (ET1601-5) at 1/2,000 dilution. Lane 1: THP-1-si NT cell lysate Lane 2: THP-1-si Beta Catenin cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-5) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Beta Catenin on different lysates with Rabbit anti-Beta Catenin antibody (ET1601-5) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Beta Catenin KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-5) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Beta Catenin
HUABIO
catalog: HA601179

mouse monoclonal (A6-F8-R)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Beta Catenin on different lysates with Mouse anti-Beta Catenin antibody (HA601179) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of MCF7 cells labeling Beta Catenin with Mouse anti-Beta Catenin antibody (HA601179) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta Catenin antibody (HA601179) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of MCF7 cells labeling Beta Catenin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601179, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1126) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 360.00 USD
to the supplier

Beta Catenin
HUABIO
catalog: HA610062

mouse monoclonal (A6-F8-R)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Beta Catenin on different lysates with Mouse anti-Beta Catenin antibody (HA610062) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610062) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of MCF7 cells labeling Beta Catenin with Mouse anti-Beta Catenin antibody (HA610062) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta Catenin antibody (HA610062) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of MCF7 cells labeling Beta Catenin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA610062, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1126) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μg
price: 649.00 USD
to the supplier

Beta Catenin
HUABIO
catalog: HA720182F

domestic rabbit monoclonal (SA30-04)
reactivity: human, mouse
application: immunocytochemistry

Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Beta Catenin (HA720182F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Beta Catenin (HA720182F, iFluor™ 488) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Immunocytochemistry analysis of HepG2 cells labeling Beta Catenin with Rabbit anti-Beta Catenin antibody (HA720182F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Beta Catenin antibody (HA720182F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 429.00 USD
to the supplier

Phospho-Beta Catenin (S33 + S37)
HUABIO
catalog: HA721580

domestic rabbit monoclonal (JE59-59)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Beta Catenin (S33 + S37) on different lysates with Rabbit anti-Phospho-Beta Catenin (S33 + S37) antibody (HA721580) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: C6 whole cell lysate Lane 4: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Beta Catenin (T41/S45)
HUABIO
catalog: HA722316

domestic rabbit monoclonal (JE54-02)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Beta Catenin (T41/S45) on different lysates with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lane 7: C6 cell lysate Lane 8: C6 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 9: C6 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 30 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: Lane 1-4: 3 minutes; Lane 5-9: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722316) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722316) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Beta Catenin (T41/S45) antibody (HA722316) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722316) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Beta Catenin (S552)
HUABIO
catalog: HA723024

domestic rabbit monoclonal (PSH08-72)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Beta Catenin (S552) on different lysates with Rabbit anti-Phospho-Beta Catenin (S552) antibody (HA723024) at 1/2,000 dilution and pan Beta Catenin antibody (ET1601-5) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM MG-132 for 6 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate Lane 5: C6 cell lysate Lane 6: HeLa treated with 10μM MG-132 for 6 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723024) at 1/2,000 dilution and pan Beta Catenin antibody (ET1601-5) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Phospho-Beta Catenin (S552) antibody (HA723024) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723024) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry analysis of C6 cells treated with 25μM MG-132 for 4 hours labeling Phospho-Beta Catenin (S552) with Rabbit anti-Phospho-Beta Catenin (S552) antibody (HA723024) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Beta Catenin (S552) antibody (HA723024) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Beta Catenin
HUABIO
catalog: HA750023

domestic rabbit monoclonal (SA30-04)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Beta Catenin on different lysates with Rabbit anti-Beta Catenin antibody (HA750023) at 1/2,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750023) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Beta Catenin on different lysates with Rabbit anti-Beta Catenin antibody (HA750023) at 1/2,000 dilution. Lane 1: THP-1-si NT cell lysate Lane 2: THP-1-si Beta Catenin cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750023) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Beta Catenin on different lysates with Rabbit anti-Beta Catenin antibody (HA750023) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Beta Catenin KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750023) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Beta Catenin (S552)
HUABIO
catalog: HA751237

domestic rabbit monoclonal (PSH08-72)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section