NG2
HUABIO
catalog: ET1703-16-50UL
domestic rabbit monoclonal (JM10-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (ET1703-16) at 1/2,000 dilution. Lane 1: A375 cell lysate (15 µg/Lane) Lane 2: SK-MEL-28 cell lysate (15 µg/Lane) Lane 3: MCF7 cell lysate (negative) (15 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 251 kDa Observed band size: 300 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-16) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (ET1703-16) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 251 kDa Observed band size: 300 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-16) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue using anti-NG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-16, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier
NG2
HUABIO
catalog: HA723167
domestic rabbit monoclonal (PSH09-98)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (HA723167) at 1/10,000 dilution. Lane 1: A375 cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: MCF7 cell lysate (negative) Lane 4: HeLa cell lysate Lane 5: PANC-1 cell lysate Lane 6: MDA-MB-231 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 251 kDa Observed band size: $330 kDa Exposure time: Lane 1-2: 6 seconds; Lane 3-6: 59 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723167) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A375 (positive) and SK-Br-3 (negative) labeling NG2 with Rabbit anti-NG2 antibody (HA723167) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NG2 antibody (HA723167) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of SK-Br-3 (left, negative) and A375 (right, positive) cells labeling NG2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723167, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
Human CSPG4
HUABIO
catalog: HA723244
domestic rabbit monoclonal (PSH10-77)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723244) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSPG4 in A-375, SK-MEL-28, SK-BR-3 and MCF7 cell culture supernatant. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curve and corrected for sample dilution. Undiluted samples are A-375 cell culture supernatant 25%, SK-MEL-28 cell culture supernatant 100%, SK-BR-3 cell culture supernatant 100% and MCF7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSPG4 concentration was determined to be 164.9 ng/ml in A-375 cell culture supernatant, 17.2 ng/ml in SK-MEL-28 cell culture supernatant and undetectable in SK-BR-3 and MCF7 cell culture supernatant.

Interpolated concentrations of spiked CSPG4 in human cell culture media samples. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CSPG4
HUABIO
catalog: HA723245
domestic rabbit monoclonal (PSH10-78)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723245) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSPG4 in A-375, SK-MEL-28, SK-BR-3 and MCF7 cell culture supernatant. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curve and corrected for sample dilution. Undiluted samples are A-375 cell culture supernatant 25%, SK-MEL-28 cell culture supernatant 100%, SK-BR-3 cell culture supernatant 100% and MCF7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSPG4 concentration was determined to be 164.9 ng/ml in A-375 cell culture supernatant, 17.2 ng/ml in SK-MEL-28 cell culture supernatant and undetectable in SK-BR-3 and MCF7 cell culture supernatant.

Interpolated concentrations of spiked CSPG4 in human cell culture media samples. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CSPG4
HUABIO
catalog: HA723246
domestic rabbit monoclonal (PSH10-79)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723244) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723245) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSPG4 in A-375, SK-MEL-28, SK-BR-3 and MCF7 cell culture supernatant. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curve and corrected for sample dilution. Undiluted samples are A-375 cell culture supernatant 25%, SK-MEL-28 cell culture supernatant 100%, SK-BR-3 cell culture supernatant 100% and MCF7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSPG4 concentration was determined to be 164.9 ng/ml in A-375 cell culture supernatant, 17.2 ng/ml in SK-MEL-28 cell culture supernatant and undetectable in SK-BR-3 and MCF7 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CSPG4
HUABIO
catalog: HA723247B
domestic rabbit monoclonal (PSH10-79)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723244) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723247B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723245) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723247B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSPG4 in A-375, SK-MEL-28, SK-BR-3 and MCF7 cell culture supernatant. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curve and corrected for sample dilution. Undiluted samples are A-375 cell culture supernatant 25%, SK-MEL-28 cell culture supernatant 100%, SK-BR-3 cell culture supernatant 100% and MCF7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSPG4 concentration was determined to be 164.9 ng/ml in A-375 cell culture supernatant, 17.2 ng/ml in SK-MEL-28 cell culture supernatant and undetectable in SK-BR-3 and MCF7 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
Human CSPG4
HUABIO
catalog: HA723376H
domestic rabbit monoclonal (PSH10-79)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723244) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA723376H, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
NG2
HUABIO
catalog: HA750372
domestic rabbit monoclonal (JM10-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (HA750372) at 1/2,000 dilution. Lane 1: A375 cell lysate (15 µg/Lane) Lane 2: SK-MEL-28 cell lysate (15 µg/Lane) Lane 3: MCF7 cell lysate (negative) (15 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 251 kDa Observed band size: 300 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750372) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (HA750372) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 251 kDa Observed band size: 300 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750372) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue using anti-NG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750372, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
NG2
HUABIO
catalog: HA751329
domestic rabbit monoclonal (PSH09-98)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of NG2 on different lysates with Rabbit anti-NG2 antibody (HA751329) at 1/10,000 dilution. Lane 1: A375 cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: MCF7 cell lysate (negative) Lane 4: HeLa cell lysate Lane 5: PANC-1 cell lysate Lane 6: MDA-MB-231 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 251 kDa Observed band size: $330 kDa Exposure time: Lane 1-2: 6 seconds; Lane 3-6: 59 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751329) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A375 (positive) and SK-Br-3 (negative) labeling NG2 with Rabbit anti-NG2 antibody (HA751329) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NG2 antibody (HA751329) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of SK-Br-3 (left, negative) and A375 (right, positive) cells labeling NG2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751329, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier