CSF-1-R
HUABIO
catalog: HA721180
domestic rabbit monoclonal (PD00-45)
reactivity: human
application: flow cytometry, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CSF-1-R antibody (HA721180) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721180) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CSF-1-R antibody (HA721180) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721180) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of SK-Br-3 cells labeling CSF-1-R. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721180, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
CSF-1-R
HUABIO
catalog: HA723142
domestic rabbit monoclonal (PSH09-77)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CSF-1-R on different lysates with Rabbit anti-CSF-1-R antibody (HA723142) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Raji cell lysate (negative) (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: J774A.1 cell lysate (20 µg/Lane) Predicted band size: 108 kDa Observed band size: 90-160 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723142) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CSF-1-R antibody (HA723142) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723142) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CSF-1-R antibody (HA723142) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723142) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Human CSF-1-R
HUABIO
catalog: HA723474
domestic rabbit monoclonal (PSH12-66)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723474) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723475, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load. Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3144 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.

Interpolated concentrations of spiked CSF1R in cell culture media samples. Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CSF-1-R
HUABIO
catalog: HA723475
domestic rabbit monoclonal (PSH12-67)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723474) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723475, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load. Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3144 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.

Interpolated concentrations of spiked CSF1R in cell culture media samples. Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CSF-1-R
HUABIO
catalog: HA723476B
domestic rabbit monoclonal (PSH12-67)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723474) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723475, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load. Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3144 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.

Interpolated concentrations of spiked CSF1R in cell culture media samples. Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human CSF-1-R
HUABIO
catalog: HA723477
domestic rabbit monoclonal (PSH12-68)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723477) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723474, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load. Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3782 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.

Interpolated concentrations of spiked CSF1R in cell culture media samples. Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CSF-1-R
HUABIO
catalog: HA723478B
domestic rabbit monoclonal (PSH12-66)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723477) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723474, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load. Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3782 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.

Interpolated concentrations of spiked CSF1R in cell culture media samples. Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human CSF-1-R
HUABIO
catalog: HA723478H
domestic rabbit monoclonal (PSH12-66)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723477) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 111,000 pg/ml to 0 pg/ml and detect antibody (HA723474, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
CSF-1-R
HUABIO
catalog: HA751319
domestic rabbit monoclonal (PSH09-77)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CSF-1-R on different lysates with Rabbit anti-CSF-1-R antibody (HA751319) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Raji cell lysate (negative) (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: J774A.1 cell lysate (20 µg/Lane) Predicted band size: 108 kDa Observed band size: 90-160 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751319) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CSF-1-R antibody (HA751319) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751319) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CSF-1-R antibody (HA751319) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751319) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier