domestic rabbit monoclonal (SU0413)
reactivity: human, mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of M-CSF on different lysates with Rabbit anti-M-CSF antibody (ET1609-1) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: THP-1 cell lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of M-CSF on different lysates with Rabbit anti-M-CSF antibody (ET1609-1) at 1/2,000 dilution. Lane 1: Mouse spleen tissue lysate (40 µg/Lane) Lane 2: Mouse colon tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of M-CSF in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH11-36)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human M-CSF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723323) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human M-CSF protein (HA210918) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA72332, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native M-CSF in MG-63 cell culture supernatant and human urine samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are MG-63 cell culture supernatant 20% and human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 16,592 pg/ml in MG-63 cell culture supernatant and 652 pg/ml in human urine samples.
Interpolated concentrations of spiked M-CSF in human cell culture media samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH11-37)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human M-CSF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723324) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human M-CSF protein (HA210918) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA72332, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native M-CSF in MG-63 cell culture supernatant and human urine samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are MG-63 cell culture supernatant 20% and human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 16,640 pg/ml in MG-63 cell culture supernatant and 738 pg/ml in human urine samples.
Interpolated concentrations of spiked M-CSF in human cell culture media samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH11-38)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human M-CSF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723323) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human M-CSF protein (HA210918) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA72332, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of human M-CSF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723324) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human M-CSF protein (HA210918) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA72332, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native M-CSF in MG-63 cell culture supernatant and human urine samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are MG-63 cell culture supernatant 20% and human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 16,592 pg/ml in MG-63 cell culture supernatant and 652 pg/ml in human urine samples.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH11-38)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human M-CSF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723323) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human M-CSF protein (HA210918) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA72336B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of human M-CSF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723324) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human M-CSF protein (HA210918) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA72336B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native M-CSF in MG-63 cell culture supernatant and human urine samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are MG-63 cell culture supernatant 20% and human urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 16,592 pg/ml in MG-63 cell culture supernatant and 652 pg/ml in human urine samples.
quantity: 100μl
price: 409.00 USD
to the supplier