domestic rabbit monoclonal (JU73-03)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of FABP3 on mouse heart tissue lysates with Rabbit anti-FABP3 antibody (ET1706-50) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-50) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-FABP3 antibody (ET1706-50) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-50) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-FABP3 antibody (ET1706-50) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-50) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (JE53-37)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of FABP3 on different lysates with Rabbit anti-FABP3 antibody (HA721859) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721859) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-FABP3 antibody (HA721859) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721859) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-FABP3 antibody (HA721859) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721859) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-98)
reactivity: human, mouse
application: ELISA
reactivity: human, mouse
application: ELISA
Sandwich ELISA analysis of Human H-FABP matched pair antibodies Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725177) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human H-FABP protein (HA211159) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725178, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native H-FABP in human serum and THP-1 cell supernatant. Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and THP-1 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 2,876 pg/ml in human serum and undetectable in THP-1 cell culture supernatant.
Interpolated concentrations of native H-FABP in mouse heart extract samples based on a 1,000 μg/ml extract load. Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are mouse heart extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 316 pg/ml in mouse heart extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-99)
reactivity: human, mouse
application: ELISA
reactivity: human, mouse
application: ELISA
Sandwich ELISA analysis of Human H-FABP matched pair antibodies Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725177) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human H-FABP protein (HA211159) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725178, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native H-FABP in human serum and THP-1 cell supernatant. Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and THP-1 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 2,876 pg/ml in human serum and undetectable in THP-1 cell culture supernatant.
Interpolated concentrations of native H-FABP in mouse heart extract samples based on a 1,000 μg/ml extract load. Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are mouse heart extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 316 pg/ml in mouse heart extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-99)
reactivity: human, mouse
conjugate: biotin
application: ELISA
reactivity: human, mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human H-FABP matched pair antibodies Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725177) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human H-FABP protein (HA211159) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725178, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native H-FABP in human serum and THP-1 cell supernatant. Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and THP-1 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 2,876 pg/ml in human serum and undetectable in THP-1 cell culture supernatant.
Interpolated concentrations of native H-FABP in mouse heart extract samples based on a 1,000 μg/ml extract load. Capture: HA725177, Human H-FABP Rabbit mAb [PSH16-98] Detector: HA725178, Human H-FABP Rabbit mAb [PSH16-99] The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curve and corrected for sample dilution. Undiluted samples are mouse heart extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 316 pg/ml in mouse heart extract.
quantity: 100μl
price: 409.00 USD
to the supplier