F4/80
HUABIO
catalog: HA721520

domestic rabbit monoclonal (PSH0-85)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate (no heat) (40 µg/Lane) Lane 2: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 4: Rat kidney tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane) Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 (F4/80, HA721520, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green) Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

F4/80
HUABIO
catalog: HA721745

domestic rabbit monoclonal (PSH01-87)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721745) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L-929 cell lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721745) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA721745) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721745) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA721745) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721745) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

F4/80
HUABIO
catalog: HA750665

domestic rabbit monoclonal (PSH0-85)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA750665) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate (no heat) (40 µg/Lane) Lane 2: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 4: Rat kidney tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750665) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA750665) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane) Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750665) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 (F4/80, HA750665, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green) Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier

F4/80
HUABIO
catalog: HA750761

domestic rabbit monoclonal (PSH01-87)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA750761) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L-929 cell lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750761) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA750761) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750761) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA750761) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750761) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

F4/80
HUABIO
catalog: IRS052

domestic rabbit monoclonal
reactivity: mouse

mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with F4/80 (IRS052), Ki67 (IRS057), CD8 and CD11c antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, F4/80 (IRS052), CD4 (IRS051), CD206 (IRS049) and CD11c antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1 (IRS059), F4/80 (IRS052), FOXP3 (IRS053), CD11c and CD4 (IRS051) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 385.00 USD
to the supplier

F4/80
HUABIO
catalog: HA601485

domestic rabbit polyclonal (PSH0-85)
reactivity: mouse, rat
application: immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rat anti-F4/80 antibody (HA601485) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601485) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rat anti-F4/80 antibody (HA601485) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601485) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

F4/80
HUABIO
catalog: HA601537

domestic rabbit polyclonal (PSH01-87)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of F4/80 on different lysates with Mouse anti-F4/80 antibody (HA601537) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (10 µg/Lane) Lane 2: L-929 cell lysate (negative) (10 µg/Lane) Lane 3: Mouse spleen tissue lysate (no heat) (10 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 150 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601537) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-F4/80 antibody (HA601537) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601537) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-F4/80 antibody (HA601537) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601537) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

F4/80
HUABIO
catalog: HA601538

domestic rabbit polyclonal (PSH01-87)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of F4/80 on mouse spleen tissue lysates with Rat anti-F4/80 antibody (HA601538) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 150 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601538) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 360.00 USD
to the supplier

F4/80
HUABIO
catalog: HA610319

domestic rabbit polyclonal (PSH0-85)
reactivity: mouse, rat
application: immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rat anti-F4/80 antibody (HA610319) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610319) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rat anti-F4/80 antibody (HA610319) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610319) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

F4/80
HUABIO
catalog: HA610356

domestic rabbit polyclonal (PSH01-87)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of F4/80 on different lysates with Mouse anti-F4/80 antibody (HA610356) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (10 µg/Lane) Lane 2: L-929 cell lysate (negative) (10 µg/Lane) Lane 3: Mouse spleen tissue lysate (no heat) (10 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 150 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610356) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-F4/80 antibody (HA610356) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610356) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-F4/80 antibody (HA610356) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610356) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

F4/80
HUABIO
catalog: HA610357

domestic rabbit polyclonal (PSH01-87)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of F4/80 on mouse spleen tissue lysates with Rat anti-F4/80 antibody (HA610357) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 150 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610357) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

F4/80
HUABIO
catalog: IRS143RT

domestic rabbit polyclonal
reactivity: mouse

mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rat anti-F4/80 antibody (IRS143RT) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900810). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 278.00 USD
to the supplier