domestic rabbit monoclonal (PSH05-02)
reactivity: human
application: flow cytometry
reactivity: human
application: flow cytometry
Immunocytochemistry analysis of HeLa cells labeling Human CD200R1 with Rabbit anti-Human CD200R1 antibody (HA722211) at 1/100 dilution. HeLa cells, transfected with empty control, CD200R1 or CD200R2 expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Human CD200R1 antibody (HA722211) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of human peripheral blood labeling Human CD200R1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722211, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-27)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human CD200R1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722256) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD200R1 protein (HA210572) starting from 3,500 pg/ml to 0 pg/ml and detect antibody (HA722257, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
The concentrations of CD200R1 were interpolated from the CD200R1 standard curves and corrected for sample dilution.Undiluted samples are mixed serum from twenty-four volunteers 25%. The mean CD200R1 concentration was determined to be 3,702 pg/mL in mixed serum from twenty-four volunteers.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-28)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human CD200R1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722256) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD200R1 protein (HA210572) starting from 3,500 pg/ml to 0 pg/ml and detect antibody (HA722257, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
The concentrations of CD200R1 were interpolated from the CD200R1 standard curves and corrected for sample dilution.Undiluted samples are mixed serum from twenty-four volunteers 25%. The mean CD200R1 concentration was determined to be 3,702 pg/mL in mixed serum from twenty-four volunteers.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-28)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA