domestic rabbit monoclonal (SU0413)
reactivity: human, mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of M-CSF on different lysates with Rabbit anti-M-CSF antibody (ET1609-1) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: THP-1 cell lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of M-CSF on different lysates with Rabbit anti-M-CSF antibody (ET1609-1) at 1/2,000 dilution. Lane 1: Mouse spleen tissue lysate (40 µg/Lane) Lane 2: Mouse colon tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of M-CSF in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-84)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse M-CSF/CSF1 matched pair antibodies Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723792) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse M-CSF/CSF1 protein (HA211182) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723793, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated RAW264.7 cell culture supernatant. Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] RAW264.7 cells were stimulated with 1 µg/ml LPS and 30 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 24 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 731.4 pg/ml in LPS and recombinant mouse ING-γ stimulated RAW264.7 cell culture supernatant and undetectable in the unstimulated RAW264.7 control.
Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated J774A.1 cell culture supernatant. Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 72 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 704.2 pg/ml in LPS and recombinant mouse ING-γ stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-85)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse M-CSF/CSF1 matched pair antibodies Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723792) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse M-CSF/CSF1 protein (HA211182) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723793, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated RAW264.7 cell culture supernatant. Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] RAW264.7 cells were stimulated with 1 µg/ml LPS and 30 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 24 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 731.4 pg/ml in LPS and recombinant mouse ING-γ stimulated RAW264.7 cell culture supernatant and undetectable in the unstimulated RAW264.7 control.
Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated J774A.1 cell culture supernatant. Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 72 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 704.2 pg/ml in LPS and recombinant mouse ING-γ stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-85)
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse M-CSF/CSF1 matched pair antibodies Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723792) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse M-CSF/CSF1 protein (HA211182) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723793, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated RAW264.7 cell culture supernatant. Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] RAW264.7 cells were stimulated with 1 µg/ml LPS and 30 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 24 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 731.4 pg/ml in LPS and recombinant mouse ING-γ stimulated RAW264.7 cell culture supernatant and undetectable in the unstimulated RAW264.7 control.
Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated J774A.1 cell culture supernatant. Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84] Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85] J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 72 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 704.2 pg/ml in LPS and recombinant mouse ING-γ stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.
quantity: 100μl
price: 409.00 USD
to the supplier