domestic rabbit polyclonal (PSH15-35)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of C Reactive Protein on different lysates with Rabbit anti-C Reactive Protein antibody (HA723739) at 1/5,000 dilution. Lane 1: Human serum tissue lysate Lane 2: Human plasma tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723739) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of C Reactive Protein on different lysates with Rabbit anti-C Reactive Protein antibody (HA723739) at 1/5,000 dilution. Lane 1: Mouse serum tissue lysate Lane 2: Mouse plasma tissue lysate Lane 3: Rat plasma tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723739) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-48)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse CRP matched pair antibodies Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725073) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CRP protein (HA211020). starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA725074, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CRP in mouse samples. Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples were 0.00625% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 92,722,639 pg/mL in mouse serum and 2,262 pg/mL in mouse heart cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-49)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse CRP matched pair antibodies Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725073) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CRP protein (HA211020). starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA725074, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CRP in mouse samples. Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples were 0.00625% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 92,722,639 pg/mL in mouse serum and 2,262 pg/mL in mouse heart cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-49)
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse CRP matched pair antibodies Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725073) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CRP protein (HA211020). starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA725074, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CRP in mouse samples. Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples were 0.00625% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 92,722,639 pg/mL in mouse serum and 2,262 pg/mL in mouse heart cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-49)
reactivity: mouse
conjugate: HRP
application: ELISA
reactivity: mouse
conjugate: HRP
application: ELISA
Sandwich ELISA analysis of Mouse CRP matched pair antibodies Capture: HA725073, Mouse CRP Rabbit mAb [PSH12-48] Detector: HA725074, Mouse CRP Rabbit mAb [PSH12-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725073) diluted in carbonate/bicarbonate buffer, at a concentration of 0.1ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CRP protein (HA211020). starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725074, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier