COL1A1
HUABIO
catalog: HA722517
domestic rabbit monoclonal (PSH06-20)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of COL1A1 on different lysates with Rabbit anti-COL1A1 antibody (HA722517) at 1/1,000 dilution. Lane 1: HFF-1 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Human lung tissue lysate (40 µg/Lane) Lane 4: Mouse skin tissue lysate (40 µg/Lane) Lane 5: Rat skin tissue lysate (40 µg/Lane) Predicted band size: 139 kDa Observed band size: 200/139 kDa Exposure time: Lane 1-4: 1 minute; Lane 5: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722517) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of COL1A1 on different lysates with Rabbit anti-COL1A1 antibody (HA722517) at 1/2,000 dilution. Lane 1: HFF-1 cell lysate (RIPA buffer) Lane 2: HFF-1 cell lysate (Hot lysis buffer) Predicted band size: 139 kDa Observed band size: 200 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722517) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fluorescence multiplex immunohistochemical analysis of human liver (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD31(M1511-8, Red), anti-COL1A1(HA722517, Magenta) and anti-αSMA (ET1607-53, Yellow) on human liver. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of M1511-8 (1/1000 dilution), HA722517 (1/10000 dilution) and ET1607-53 (1/5000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95C. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 385.00 USD
to the supplier
Mouse Pro-Collagen I alpha 1
HUABIO
catalog: HA722934
domestic rabbit monoclonal (PSH07-98)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse Pro-Collagen I alpha 1 matched pair antibodies Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722934) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722935, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse samples. Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 2146 ng/mL in mouse serum, 170 ng/ml in NIH/3T3 and 10 ng/ml mouse lung cell culture supernatant.

Interpolated concentrations of spiked Pro‑Collagen I alpha 1 in cell culture media samples. Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] The concentrations of Pro‑Collagen I alpha 1 were measured in duplicates, interpolated from the Pro‑Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse Pro-Collagen I alpha 1
HUABIO
catalog: HA722935
domestic rabbit monoclonal (PSH07-99)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse Pro-Collagen I alpha 1 matched pair antibodies Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722934) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722935, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse samples. Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 2146 ng/mL in mouse serum, 170 ng/ml in NIH/3T3 and 10 ng/ml mouse lung cell culture supernatant.

Interpolated concentrations of spiked Pro‑Collagen I alpha 1 in cell culture media samples. Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] The concentrations of Pro‑Collagen I alpha 1 were measured in duplicates, interpolated from the Pro‑Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse Pro-Collagen I alpha 1
HUABIO
catalog: HA722936B
domestic rabbit monoclonal (PSH07-99)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of mouse Pro-Collagen I alpha 1 matched pair antibodies Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722934) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722935, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse samples. Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 2146 ng/mL in mouse serum, 170 ng/ml in NIH/3T3 and 10 ng/ml mouse lung cell culture supernatant.

Interpolated concentrations of spiked Pro‑Collagen I alpha 1 in cell culture media samples. Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] The concentrations of Pro‑Collagen I alpha 1 were measured in duplicates, interpolated from the Pro‑Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Mouse Pro-Collagen I alpha 1
HUABIO
catalog: HA722936H
domestic rabbit monoclonal (PSH07-99)
reactivity: mouse
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of mouse Pro-Collagen I alpha 1 matched pair antibodies Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98] Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722934) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA722935, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
COL1A1
HUABIO
catalog: HA751058
domestic rabbit monoclonal (PSH06-20)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of COL1A1 on different lysates with Rabbit anti-COL1A1 antibody (HA751058) at 1/1,000 dilution. Lane 1: HFF-1 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Human lung tissue lysate (40 µg/Lane) Lane 4: Mouse skin tissue lysate (40 µg/Lane) Lane 5: Rat skin tissue lysate (40 µg/Lane) Predicted band size: 139 kDa Observed band size: 200/139 kDa Exposure time: Lane 1-4: 1 minute; Lane 5: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751058) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of COL1A1 on different lysates with Rabbit anti-COL1A1 antibody (HA751058) at 1/2,000 dilution. Lane 1: HFF-1 cell lysate (RIPA buffer) Lane 2: HFF-1 cell lysate (Hot lysis buffer) Predicted band size: 139 kDa Observed band size: 200 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751058) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HFF-1 cells labeling COL1A1 with Rabbit anti-COL1A1 antibody (HA751058) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COL1A1 antibody (HA751058) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
COL1A1
HUABIO
catalog: IRS228RB
domestic rabbit monoclonal
reactivity: mouse

mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-COL1A1 antibody (IRS228RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 278.00 USD
to the supplier