Creatine kinase B type
HUABIO
catalog: ET7107-52
domestic rabbit monoclonal (JB78-34)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (ET7107-52) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Y79 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-52) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (ET7107-52) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Creatine kinase B type KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-52) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Creatine kinase B type antibody (ET7107-52) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Creatine kinase B type
HUABIO
catalog: HA723090
domestic rabbit monoclonal (PSH09-32)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Y79 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse cerebellum tissue lysate Lane 6: Rat cerebellum tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723090) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SH-SY5Y cells labeling Creatine kinase B type with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Creatine kinase B type antibody (HA723090) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of SH-SY5Y cells labeling Creatine kinase B type. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723090, 1/200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/200dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
Human CKMB
HUABIO
catalog: HA723348
domestic rabbit monoclonal (PSH11-55)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of human CKMB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723348) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CKMB protein (HA210947) starting from 80000 pg/ml to 0 pg/ml and detect antibody (HA723349, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CKMB in rat heart and rat skeletal muscle extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CKMB was measured in duplicate at different sample concentrations and interpolated from the CKMB standard curves. The mean CKMB concentration was determined to be 62,193 pg/mL in rat heart tissue extract. There was no detectable signal in rat skeletal muscle extract.

Interpolated concentrations of spiked CKMB in cell culture media samples. The concentrations of CKMB were measured in duplicates, interpolated from the CKMB standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CKMB
HUABIO
catalog: HA723349
domestic rabbit monoclonal (PSH11-56)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of human CKMB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723348) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CKMB protein (HA210947) starting from 80000 pg/ml to 0 pg/ml and detect antibody (HA723349, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CKMB in rat heart and rat skeletal muscle extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CKMB was measured in duplicate at different sample concentrations and interpolated from the CKMB standard curves. The mean CKMB concentration was determined to be 62,193 pg/mL in rat heart tissue extract. There was no detectable signal in rat skeletal muscle extract.

Interpolated concentrations of spiked CKMB in cell culture media samples. The concentrations of CKMB were measured in duplicates, interpolated from the CKMB standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CKMB
HUABIO
catalog: HA723350B
domestic rabbit monoclonal (PSH11-56)
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CKMB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723348) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CKMB protein (HA210777) starting from 80000 pg/ml to 0 pg/ml and detect antibody (HA723350B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CKMB in rat heart and rat skeletal muscle extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native CKMB was measured in duplicate at different sample concentrations and interpolated from the CKMB standard curves. The mean CKMB concentration was determined to be 62,193 pg/mL in rat heart tissue extract. There was no detectable signal in rat skeletal muscle extract.

Interpolated concentrations of spiked CKMB in cell culture media samples. The concentrations of CKMB were measured in duplicates, interpolated from the CKMB standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Creatine kinase B type
HUABIO
catalog: HA751286
domestic rabbit monoclonal (PSH09-32)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (HA751286) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Y79 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse cerebellum tissue lysate Lane 6: Rat cerebellum tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751286) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SH-SY5Y cells labeling Creatine kinase B type with Rabbit anti-Creatine kinase B type antibody (HA751286) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Creatine kinase B type antibody (HA751286) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of SH-SY5Y cells labeling Creatine kinase B type. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751286, 1/200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/200dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier