CD86
HUABIO
catalog: ET1606-50-50UL
domestic rabbit monoclonal (SJ20-00)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/5,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lane 4: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 38 kDa Observed band size: 70 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/5,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane) Lane 2: Mouse lung tissue lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 38 kDa Observed band size: 70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody (ET1606-50) at 1/500 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody (ET1606-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 50μl
price: 205.00 USD
to the supplier
Mouse CD86
HUABIO
catalog: HA723552
domestic rabbit monoclonal (PSH13-63)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse CD86 matched pair antibodies Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723552) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (HA210879) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723553, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD86 in mouse serum samples. Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 999 pg/mL in Mouse serum.

Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 220 pg/mL in J774A.1 Cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse CD86
HUABIO
catalog: HA723553
domestic rabbit monoclonal (PSH13-64)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse CD86 matched pair antibodies Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723552) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (HA210879) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723553, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD86 in mouse serum samples. Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 999 pg/mL in Mouse serum.

Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 220 pg/mL in J774A.1 Cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse CD86
HUABIO
catalog: HA723554B
domestic rabbit monoclonal (PSH13-64)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse CD86 matched pair antibodies Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723552) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (HA210879) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723553, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD86 in mouse serum samples. Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 999 pg/mL in Mouse serum.

Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723552, Mouse CD86 Rabbit mAb [PSH13-63] Detector: HA723553, Mouse CD86 Rabbit mAb [PSH13-64] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 220 pg/mL in J774A.1 Cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier
Mouse CD86
HUABIO
catalog: HA723555
domestic rabbit monoclonal (PSH13-65)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse CD86 matched pair antibodies Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723555) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (HA210879) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723556, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD86 in mouse serum samples. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 263 pg/mL in Mouse serum.

Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 181 pg/mL in J774A.1 Cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse CD86
HUABIO
catalog: HA723556
domestic rabbit monoclonal (PSH13-66)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse CD86 matched pair antibodies Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723555) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (HA210879) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723556, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD86 in mouse serum samples. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 263 pg/mL in Mouse serum.

Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 181 pg/mL in J774A.1 Cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse CD86
HUABIO
catalog: HA723557B
domestic rabbit monoclonal (PSH13-66)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse CD86 matched pair antibodies Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723555) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD86 protein (HA210879) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723556, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD86 in mouse serum samples. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 263 pg/mL in Mouse serum.

Interpolated concentrations of native CD68 in J774A.1 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723555, Mouse CD86 Rabbit mAb [PSH13-65] Detector: HA723556, Mouse CD86 Rabbit mAb [PSH13-66] Interpolated concentration of native CD86 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD86 concentration was determined to be 181 pg/mL in J774A.1 Cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier
CD86
HUABIO
catalog: HA750109
domestic rabbit monoclonal (SJ20-00)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (HA750109) at 1/5,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lane 4: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 38 kDa Observed band size: 70 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750109) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (HA750109) at 1/5,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane) Lane 2: Mouse lung tissue lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 38 kDa Observed band size: 70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750109) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody (HA750109) at 1/500 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody (HA750109) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
Anti-CD86 Antibody [SJ20-00]
HUABIO
catalog: ET1606-50TR
domestic rabbit monoclonal (SJ20-00)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry
quantity: 20 uL
price: 99 USD
to the supplier