CD68
HUABIO
catalog: HA722285

domestic rabbit monoclonal (PSH05-47)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-CD68 antibody (HA722285) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722285, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen mouse liver tissue with Rabbit anti-CD68 antibody (HA722285) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722285, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen rat liver tissue with Rabbit anti-CD68 antibody (HA722285) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722285, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier

CD68
HUABIO
catalog: HA722737

domestic rabbit monoclonal (PSH06-65)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: M‑NFS‑60 cell lysate (20 µg/Lane) Lane 3: J774A.1 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 5: Neuro-2a cell lysate (negative) (20 µg/Lane) Lane 6: Rat spleen tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 35 kDa Observed band size: 90-200 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 60-100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

Mouse CD68
HUABIO
catalog: HA723446

domestic rabbit monoclonal (PSH12-24)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse CD68 matched pair antibodies Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723446) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (HA211131) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723447, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load. Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 44257 pg/mL in mouse spleen extract,106874 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract.

Interpolated concentrations of spiked CD68 in cell culture media samples. Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse CD68
HUABIO
catalog: HA723447

domestic rabbit monoclonal (PSH12-25)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse CD68 matched pair antibodies Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723446) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (HA211131) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723447, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load. Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 44257 pg/mL in mouse spleen extract,106874 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract.

Interpolated concentrations of spiked CD68 in cell culture media samples. Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse CD68
HUABIO
catalog: HA723448B

domestic rabbit monoclonal (PSH12-25)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse CD68 matched pair antibodies Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723446) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (HA211131) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723447, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load. Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 44257 pg/mL in mouse spleen extract,106874 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract.

Interpolated concentrations of spiked CD68 in cell culture media samples. Capture: HA723446, Mouse CD68 Rabbit mAb [PSH12-24] Detector: HA723447, Mouse CD68 Rabbit mAb [PSH12-25] The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse CD68
HUABIO
catalog: HA723449

domestic rabbit monoclonal (PSH12-26)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse CD68 matched pair antibodies Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723449) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (HA211131) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723450, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load. Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 35353 pg/mL in mouse spleen extract,47421 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract.

Interpolated concentrations of spiked CD68 in cell culture media samples. Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse CD68
HUABIO
catalog: HA723450

domestic rabbit monoclonal (PSH12-27)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse CD68 matched pair antibodies Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723449) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (HA211131) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723450, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load. Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 35353 pg/mL in mouse spleen extract,47421 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract.

Interpolated concentrations of spiked CD68 in cell culture media samples. Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse CD68
HUABIO
catalog: HA723451B

domestic rabbit monoclonal (PSH12-27)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse CD68 matched pair antibodies Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723449) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD68 protein (HA211131) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723450, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD68 in mouse spleen, Raw264.7 and Neuro-2a extract samples based on a 1000 µg/ml extract load. Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD68 concentration was determined to be 35353 pg/mL in mouse spleen extract,47421 pg/ml in Raw264.7 extract and undetectable in Neuro-2a extract.

Interpolated concentrations of spiked CD68 in cell culture media samples. Capture: HA723449, Mouse CD68 Rabbit mAb [PSH12-26] Detector: HA723450, Mouse CD68 Rabbit mAb [PSH12-27] The concentrations of CD68 were measured in duplicates, interpolated from the CD68 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

CD68
HUABIO
catalog: HA751006

domestic rabbit monoclonal (PSH05-47)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-CD68 antibody (HA751006) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751006, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen mouse liver tissue with Rabbit anti-CD68 antibody (HA751006) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751006, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen rat liver tissue with Rabbit anti-CD68 antibody (HA751006) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751006, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 649.00 USD
to the supplier

CD68
HUABIO
catalog: HA751094

domestic rabbit monoclonal (PSH06-65)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA751094) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: M‑NFS‑60 cell lysate (20 µg/Lane) Lane 3: J774A.1 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 5: Neuro-2a cell lysate (negative) (20 µg/Lane) Lane 6: Rat spleen tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 35 kDa Observed band size: 90-200 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751094) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA751094) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 60-100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751094) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier