Clusterin
HUABIO
catalog: ER2001-58
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Clusterin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SKBR-3 cell lysate

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Clusterin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-58, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Clusterin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-58, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
Clusterin
HUABIO
catalog: HA722116
domestic rabbit monoclonal (PSH04-33)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Clusterin on different lysates with Rabbit anti-Clusterin antibody (HA722116) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SK-Br-3 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: PANC-1 cell lysate (20 µg/Lane) Lane 5: MCF7 cell lysate (20 µg/Lane) Lane 6: T-47D cell lysate (20 µg/Lane) Predicted band size: 52 kDa Observed band size: 35-80 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722116) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IF-Tissue Species: Human Site: pancreas Sample: Paraffin-embedded section Antibody concentration: 1/2,000

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Clusterin antibody (HA722116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Human Clusterin
HUABIO
catalog: HA725091
domestic rabbit monoclonal (PSH15-55)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Clusterin matched pair antibodies Capture: HA725091, Human Clusterin Rabbit mAb [PSH15-55] Detector: HA725092, Human Clusterin Rabbit mAb [PSH15-56] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725091) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Clusterin protein (HA210955) starting from 1000,000 pg/ml to 0 pg/ml and detect antibody (HA725092, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
Human Clusterin
HUABIO
catalog: HA725092
domestic rabbit monoclonal (PSH15-56)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Clusterin matched pair antibodies Capture: HA725091, Human Clusterin Rabbit mAb [PSH15-55] Detector: HA725092, Human Clusterin Rabbit mAb [PSH15-56] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725091) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Clusterin protein (HA210955) starting from 1000,000 pg/ml to 0 pg/ml and detect antibody (HA725092, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
Human Clusterin
HUABIO
catalog: HA725093B
domestic rabbit monoclonal (PSH15-56)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Clusterin matched pair antibodies Capture: HA725091, Human Clusterin Rabbit mAb [PSH15-55] Detector: HA725092, Human Clusterin Rabbit mAb [PSH15-56] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725091) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Clusterin protein (HA210955) starting from 1000,000 pg/ml to 0 pg/ml and detect antibody (HA725092, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
Human Clusterin
HUABIO
catalog: HA725093H
domestic rabbit monoclonal (PSH15-56)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human Clusterin matched pair antibodies Capture: HA725091, Human Clusterin Rabbit mAb [PSH15-55] Detector: HA725092, Human Clusterin Rabbit mAb [PSH15-56] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725091) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Clusterin protein (HA210955) starting from 1000,000 pg/ml to 0 pg/ml and detect antibody (HA725092, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier