mouse monoclonal (11F1)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (EM1701-87) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-87) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (EM1701-87) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ASS1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-87) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining ASS1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 360.00 USD
to the supplier
mouse monoclonal (11F1-RA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA601101) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: Human liver tissue lysate (30 µg/Lane) Lane 3: Human kidney tissue lysate (30 µg/Lane) Lane 4: Mouse liver tissue lysate (30 µg/Lane) Lane 5: Mouse kidney tissue lysate (30 µg/Lane) Lane 6: Rat liver tissue lysate (30 µg/Lane) Lane 7: Rat kidney tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601101) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA601101) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ASS1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601101) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ASS1 antibody (HA601101) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601101) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
mouse monoclonal (11F1-RA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA610025) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: Human liver tissue lysate (30 µg/Lane) Lane 3: Human kidney tissue lysate (30 µg/Lane) Lane 4: Mouse liver tissue lysate (30 µg/Lane) Lane 5: Mouse kidney tissue lysate (30 µg/Lane) Lane 6: Rat liver tissue lysate (30 µg/Lane) Lane 7: Rat kidney tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610025) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA610025) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ASS1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610025) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ASS1 antibody (HA610025) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610025) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of ASS1 on Hela (1) and MCF-7 (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.
ICC staining ASS1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ASS1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier