HUABIO Abeta antibody

Amyloid Beta A4 Precursor (APP)
HUABIO
catalog: 1007-5

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Amyloid Beta A4 Precursor (APP) on different lysates with Rabbit anti-Amyloid Beta A4 Precursor (APP) antibody (1007-5) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HUVEC cell lysate Lane 3: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1007-5) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

ICC on D3.
quantity: 100μl
price: 330 USD
to the supplier

beta Amyloid 1-40
HUABIO
catalog: HA721788

domestic rabbit monoclonal (PSH02-82)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (HA721788) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721788) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (HA721788) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721788) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

beta Amyloid 1-42
HUABIO
catalog: HA721789

domestic rabbit monoclonal (PSH02-83)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721789) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721789) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

beta Amyloid 1-40
HUABIO
catalog: HA722077

domestic rabbit monoclonal (PSH02-82)
reactivity: human, mouse
application: ELISA

Sandwich ELISA analysis of Human Aβ40 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722077 diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human Aβ40 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody HA722078-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse Aβ40 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722077) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse Aβ40 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722078, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Aβ37, Aβ38, Aβ39, Aβ40, Aβ41 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722077) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Aβ37, Aβ38, Aβ39, Aβ40, Aβ41 protein at a concentration of 2000 pg/ml and detect antibody (HA722078, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

beta Amyloid 1-40
HUABIO
catalog: HA722078

domestic rabbit monoclonal (PSH03-98)
reactivity: human, mouse
application: ELISA

Sandwich ELISA analysis of Human Aβ40 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722077 diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human Aβ40 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody HA722078-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse Aβ40 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722077) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse Aβ40 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722078, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Aβ37, Aβ38, Aβ39, Aβ40, Aβ41 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722077) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Aβ37, Aβ38, Aβ39, Aβ40, Aβ41 protein at a concentration of 2000 pg/ml and detect antibody (HA722078, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

APP/beta Amyloid
HUABIO
catalog: HA722139

domestic rabbit monoclonal (JE30-54)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Western blot analysis of APP/beta Amyloid on different lysates with Rabbit anti-APP/beta Amyloid antibody (HA722139) at 1/5,000 dilution. Lane 1: PC-3 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-APP/beta Amyloid antibody (HA722139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

APP/beta Amyloid
HUABIO
catalog: HA722207

domestic rabbit monoclonal (PSH04-90)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of APP/beta Amyloid on different lysates with Rabbit anti-APP/beta Amyloid antibody (HA722207) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 87-120 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of APP/beta Amyloid on different lysates with Rabbit anti-APP/beta Amyloid antibody (HA722207) at 1/1,000 dilution. Lane 1: AD Mouse brain tissue lysate Lane 2: Mouse brain tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells treated with 100ng/mL Nocodazole for 17 hours labeling APP/beta Amyloid with Rabbit anti-APP/beta Amyloid antibody (HA722207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-APP/beta Amyloid antibody (HA722207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier

beta Amyloid 1-40
HUABIO
catalog: HA722485

domestic rabbit monoclonal (PSH05-86)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (HA722485) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722485) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded normal mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (HA722485) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722485) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

beta Amyloid 1-40
HUABIO
catalog: HA722618B

domestic rabbit monoclonal (PSH03-98)
reactivity: human, mouse
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

beta Amyloid 1-42
HUABIO
catalog: HA722754

domestic rabbit monoclonal (PSH06-81)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-42 antibody (HA722754) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722754) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-42 antibody (HA722754) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722754) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

beta Amyloid
HUABIO
catalog: HA722961

domestic rabbit monoclonal (PSH08-18)
reactivity: human, mouse
application: western blot, dot blot

beta Amyloid
HUABIO
catalog: HA722962

domestic rabbit monoclonal (PSH08-19)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, dot blot

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid antibody (HA722962) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722962) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid antibody (HA722962) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722962) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid with Rabbit anti-beta Amyloid antibody (HA722962) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722962, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier

beta Amyloid 1-40
HUABIO
catalog: HA750789

domestic rabbit monoclonal (PSH02-82)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (HA750789) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750789) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (HA750789) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750789) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

beta Amyloid 1-42
HUABIO
catalog: HA750790

domestic rabbit monoclonal (PSH02-83)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-42 antibody (HA750790) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750790) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-42 antibody (HA750790) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750790) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

APP/beta Amyloid
HUABIO
catalog: HA750977

domestic rabbit monoclonal (PSH04-90)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of APP/beta Amyloid on different lysates with Rabbit anti-APP/beta Amyloid antibody (HA750977) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 87-120 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750977) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of APP/beta Amyloid on different lysates with Rabbit anti-APP/beta Amyloid antibody (HA750977) at 1/1,000 dilution. Lane 1: AD Mouse brain tissue lysate Lane 2: Mouse brain tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750977) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells treated with 100ng/mL Nocodazole for 17 hours labeling APP/beta Amyloid with Rabbit anti-APP/beta Amyloid antibody (HA750977) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-APP/beta Amyloid antibody (HA750977) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 649.00 USD
to the supplier

beta Amyloid 1-40
HUABIO
catalog: HA751034

domestic rabbit monoclonal (PSH05-86)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded normal mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-40 antibody (HA751034) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751034) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

beta Amyloid 1-42
HUABIO
catalog: HA751103

domestic rabbit monoclonal (PSH06-81)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-42 antibody (HA751103) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751103) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-42 antibody (HA751103) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751103) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

beta Amyloid
HUABIO
catalog: HA751209

domestic rabbit monoclonal (PSH08-18)
reactivity: human, mouse
application: western blot, dot blot

beta Amyloid
HUABIO
catalog: HA751210

domestic rabbit monoclonal (PSH08-19)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, dot blot

Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid antibody (HA751210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid antibody (HA751210) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751210) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid with Rabbit anti-beta Amyloid antibody (HA751210) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751210, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 649.00 USD
to the supplier