Apolipoprotein E
HUABIO
catalog: HA723216
domestic rabbit monoclonal (PSH10-52)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Apolipoprotein E antibody (HA723216) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723216, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-Apolipoprotein E antibody (HA723216) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723216, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Apolipoprotein E antibody (HA723216) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723216) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Mouse Apolipoprotein E
HUABIO
catalog: HA725221
domestic rabbit monoclonal (PSH16-83)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse Apolipoprotein E matched pair antibodies Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83] Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725221) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein E protein (HA211210) starting from 200,000 pg/ml to 2.0 pg/ml and detect antibody (HA725222, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native APOE in mouse liver, C2C12 extract samples based on a 1mg/ml extract load. Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83] Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84] The concentrations of APOE were measured in duplicates, interpolated from the APOE standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOE concentration was determined to be 813 ng/ml in mouse liver extract, There was no detected signal in C2C12 cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse Apolipoprotein E
HUABIO
catalog: HA725222
domestic rabbit monoclonal (PSH16-84)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse Apolipoprotein E matched pair antibodies Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83] Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725221) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein E protein (HA211210) starting from 200,000 pg/ml to 2.0 pg/ml and detect antibody (HA725222, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native APOE in mouse liver, C2C12 extract samples based on a 1mg/ml extract load. Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83] Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84] The concentrations of APOE were measured in duplicates, interpolated from the APOE standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOE concentration was determined to be 813 ng/ml in mouse liver extract, There was no detected signal in C2C12 cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse Apolipoprotein E
HUABIO
catalog: HA725222B
domestic rabbit monoclonal (PSH16-84)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse Apolipoprotein E matched pair antibodies Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83] Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725221) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Apolipoprotein E protein (HA211210) starting from 200,000 pg/ml to 2.0 pg/ml and detect antibody (HA725222, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native APOE in mouse liver, C2C12 extract samples based on a 1mg/ml extract load. Capture: HA725221, Mouse Apolipoprotein E Rabbit mAb [PSH16-83] Detector: HA725222, Mouse Apolipoprotein E Rabbit mAb [PSH16-84] The concentrations of APOE were measured in duplicates, interpolated from the APOE standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOE concentration was determined to be 813 ng/ml in mouse liver extract, There was no detected signal in C2C12 cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier
Apolipoprotein E
HUABIO
catalog: HA751355
domestic rabbit monoclonal (PSH10-52)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Apolipoprotein E antibody (HA751355) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751355, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-Apolipoprotein E antibody (HA751355) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751355, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Apolipoprotein E antibody (HA751355) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751355) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
Apolipoprotein E
HUABIO
catalog: HA601391
domestic rabbit polyclonal (PSH10-52)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse brain tissue with Rat anti-Apolipoprotein E antibody (HA601391) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601391, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Apolipoprotein E antibody (HA601391) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601391) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Apolipoprotein E antibody (HA601391) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601391) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
Apolipoprotein E
HUABIO
catalog: HA610233
domestic rabbit polyclonal (PSH10-52)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse brain tissue with Rat anti-Apolipoprotein E antibody (HA610233) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610233, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Apolipoprotein E antibody (HA610233) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610233) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Apolipoprotein E antibody (HA610233) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610233) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier