Rat IL-2
HUABIO
catalog: HA721612

domestic rabbit monoclonal (PS01-88)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of Rat IL-2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721612) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IL-2 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721613) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Rat IL-2
HUABIO
catalog: HA721613

domestic rabbit monoclonal (PS01-89)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of Rat IL-2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721612) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IL-2 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721613) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Rat IL-2
HUABIO
catalog: HA721614

domestic rabbit monoclonal (PS01-89)
reactivity: rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Rat IL-2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721612) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IL-2 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721614) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Rat IL-2
HUABIO
catalog: HA722423

domestic rabbit monoclonal (PSH05-71)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of rat IL-2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722423) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted recombinant standard Rat IL-2 protein (HA210683) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722423, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen(18 hours) supernatant samples. Rat spleen was cultured for 18 hours in the presence or absence of 3 µg/ml Concanavalin A. The concentrations of IL-2 measured in duplicate and interpolated from the IL-2 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 8% and stimulated 8%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 4,659 pg/ml in Concanavalin A stimulated rat spleen supernatant and undetectable in unstimulated rat spleen supernatant.

Interpolated concentrations of spiked IL-2 in rat cell culture media samples. The concentrations of IL-2 were interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.
quantity: 100μl
price: 649.00 USD
to the supplier

Rat IL-2
HUABIO
catalog: HA722424

domestic rabbit monoclonal (PSH05-72)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of rat IL-2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722423) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted recombinant standard Rat IL-2 protein (HA210683) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722423, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen(18 hours) supernatant samples. Rat spleen was cultured for 18 hours in the presence or absence of 3 µg/ml Concanavalin A. The concentrations of IL-2 measured in duplicate and interpolated from the IL-2 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 8% and stimulated 8%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 4,659 pg/ml in Concanavalin A stimulated rat spleen supernatant and undetectable in unstimulated rat spleen supernatant.

Interpolated concentrations of spiked IL-2 in rat cell culture media samples. The concentrations of IL-2 were interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.
quantity: 100μl
price: 649.00 USD
to the supplier

Rat IL-2
HUABIO
catalog: HA722590B

domestic rabbit monoclonal (PSH05-72)
reactivity: rat
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier