domestic rabbit monoclonal (PSH17-09)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse Angiotensinogen/AGT matched pair antibodies Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725131) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Angiotensinogen/AGT protein (HA211120) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725132, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native AGT in mouse serum samples. Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse serum 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 12.78 µg/ml in mouse serum.
Interpolated concentrations of native AGT in mouse liver lysate samples. Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse liver supernatant 0.2mg/ml. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 72.92 ng/ml in mouse liver lysate.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-10)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse Angiotensinogen/AGT matched pair antibodies Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725131) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Angiotensinogen/AGT protein (HA211120) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725132, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native AGT in mouse serum samples. Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse serum 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 12.78 µg/ml in mouse serum.
Interpolated concentrations of native AGT in mouse liver lysate samples. Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse liver supernatant 0.2mg/ml. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 72.92 ng/ml in mouse liver lysate.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-10)
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse Angiotensinogen/AGT matched pair antibodies Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725131) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Angiotensinogen/AGT protein (HA211120) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725132, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native AGT in mouse serum samples. Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse serum 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 12.78 µg/ml in mouse serum.
Interpolated concentrations of native AGT in mouse liver lysate samples. Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse liver supernatant 0.2mg/ml. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 72.92 ng/ml in mouse liver lysate.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-10)
reactivity: mouse
conjugate: HRP
application: ELISA
reactivity: mouse
conjugate: HRP
application: ELISA
Sandwich ELISA analysis of Mouse Angiotensinogen/AGT matched pair antibodies Capture: HA725131, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-09] Detector: HA725132, Mouse Angiotensinogen/AGT Rabbit mAb [PSH17-10] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725131) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Angiotensinogen/AGT protein (HA211120) starting from 333,000 pg/ml to 0 pg/ml and detect antibody (HA725132, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier