NLRP3
HUABIO
catalog: ER1706-72
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NLRP3 on LOVO cell lysates with Rabbit anti-NLRP3 antibody (ER1706-72) at 1/500 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 118 kDa Observed band size: 118 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

ICC staining NLRP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining NLRP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier
NLRP3
HUABIO
catalog: ET1610-93-50UL
domestic rabbit monoclonal (SC06-23)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 118 kDa Observed band size: 118 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-93) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of RAW264.7 cells treated with 10μg/mL LPS for 8 hours labeling NLRP3 with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of HUVEC cells labeling NLRP3 with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 50μl
price: 205.00 USD
to the supplier
Human NLRP3
HUABIO
catalog: HA723275
domestic rabbit monoclonal (PSH11-07)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human NLRP3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723275) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NLRP3 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723276, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NLRP3 in THP-1 and Jurkat extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NLRP3 was measured in duplicate at different sample concentrations and interpolated from the NLRP3 standard curves. The mean NLRP3 concentration was determined to be 691 pg/mL in THP-1 cell extract. There was no detectable signal in Jurkat cell extract.

Interpolated concentrations of spiked NLRP3 in cell culture media samples. The concentrations of NLRP3 were measured in duplicates, interpolated from the NLRP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human NLRP3
HUABIO
catalog: HA723276
domestic rabbit monoclonal (PSH11-08)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human NLRP3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723275) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NLRP3 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723276, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NLRP3 in THP-1 and Jurkat extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NLRP3 was measured in duplicate at different sample concentrations and interpolated from the NLRP3 standard curves. The mean NLRP3 concentration was determined to be 691 pg/mL in THP-1 cell extract. There was no detectable signal in Jurkat cell extract.

Interpolated concentrations of spiked NLRP3 in cell culture media samples. The concentrations of NLRP3 were measured in duplicates, interpolated from the NLRP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human NLRP3
HUABIO
catalog: HA723277B
domestic rabbit monoclonal (PSH11-08)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human NLRP3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723275) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NLRP3 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723277B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NLRP3 in THP-1 and Jurkat extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NLRP3 was measured in duplicate at different sample concentrations and interpolated from the NLRP3 standard curves. The mean NLRP3 concentration was determined to be 691 pg/mL in THP-1 cell extract. There was no detectable signal in Jurkat cell extract.

Interpolated concentrations of spiked NLRP3 in cell culture media samples. The concentrations of NLRP3 were measured in duplicates, interpolated from the NLRP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human NLRP3
HUABIO
catalog: HA723278
domestic rabbit monoclonal (PSH11-09)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human NLRP3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723275) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NLRP3 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723278, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NLRP3 in THP-1 and Jurkat extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NLRP3 was measured in duplicate at different sample concentrations and interpolated from the NLRP3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NLRP3 concentration was determined to be 888 pg/mL in THP-1 cell extract. There was no detectable signal in Jurkat cell extract.

Interpolated concentrations of spiked NLRP3 in cell culture media samples. The concentrations of NLRP3 were measured in duplicates, interpolated from the NLRP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human NLRP3
HUABIO
catalog: HA723279B
domestic rabbit monoclonal (PSH11-09)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human NLRP3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723275) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NLRP3 protein starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723279B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NLRP3 in THP-1 and Jurkat extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NLRP3 was measured in duplicate at different sample concentrations and interpolated from the NLRP3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NLRP3 concentration was determined to be 888 pg/mL in THP-1 cell extract. There was no detectable signal in Jurkat cell extract.

Interpolated concentrations of spiked NLRP3 in cell culture media samples. The concentrations of NLRP3 were measured in duplicates, interpolated from the NLRP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
NLRP3
HUABIO
catalog: HA750236
domestic rabbit monoclonal (SC06-23)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA750236) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 118 kDa Observed band size: 118 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750236) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of RAW264.7 cells treated with 10μg/mL LPS for 8 hours labeling NLRP3 with Rabbit anti-NLRP3 antibody (HA750236) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody (HA750236) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of HUVEC cells labeling NLRP3 with Rabbit anti-NLRP3 antibody (HA750236) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody (HA750236) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 649.00 USD
to the supplier
Anti-NLRP3 Antibody [SC06-23]
HUABIO
catalog: ET1610-93TR
domestic rabbit monoclonal (SC06-23)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 20 uL
price: 99 USD
to the supplier