Adiponectin
HUABIO
catalog: HA721064

domestic rabbit monoclonal (JE65-49)
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (HA721064) at 1/2,000 dilution. Lane 1: Mouse white adipose lysate Lane 2: Mouse brown adipose tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721064) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (HA721064) at 1/2,000 dilution. Lane 1: Mouse serum lysate Lane 2: Mouse plasma lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721064) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of NCI-H441 cells labeling Adiponectin with Rabbit anti-Adiponectin antibody (HA721064) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Adiponectin antibody (HA721064) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Adiponectin
HUABIO
catalog: HA722286

domestic rabbit monoclonal (PSH05-48)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (HA722286) at 1/2,000 dilution. Lane 1: Mouse white adipose tissue lysate Lane 2: Rat white adipose tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722286) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Adiponectin on human plasma lysates with Rabbit anti-Adiponectin antibody (HA722286) at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722286) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Adiponectin antibody (HA722286) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722286) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Mouse Adiponectin
HUABIO
catalog: HA723679

domestic rabbit monoclonal (PSH14-82)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse Adiponectin matched pair antibodies Capture: HA723679, Mouse Adiponectin Rabbit mAb [PSH14-82] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723679) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Adiponectin protein (HA210973) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723680, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Adiponectin in mouse samples. Capture: HA723679, Mouse Adiponectin Rabbit mAb [PSH14-82] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Interpolated concentration of native Adiponectin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 32 ug/mL in mouse serum and 49 ng/ml in mouse lung cell supernatant. There was no detectable signal in NIH/3T3 cell culture supernatant.

Interpolated concentrations of spiked Adiponectin in cell culture media samples. Capture: HA723679, Mouse Adiponectin Rabbit mAb [PSH14-82] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse Adiponectin
HUABIO
catalog: HA723680

domestic rabbit monoclonal (PSH14-83)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse Adiponectin matched pair antibodies Capture: HA723679, Mouse Adiponectin Rabbit mAb [PSH14-82] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723679) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Adiponectin protein (HA210973) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723680, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of mouse Adiponectin matched pair antibodies Capture: HA723682, Mouse Adiponectin Rabbit mAb [PSH14-84] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723682) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Adiponectin protein (HA210973) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723680, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Adiponectin in mouse samples. Capture: HA723679, Mouse Adiponectin Rabbit mAb [PSH14-82] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Interpolated concentration of native Adiponectin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 32 ug/mL in mouse serum and 49 ng/ml in mouse lung cell supernatant. There was no detectable signal in NIH/3T3 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse Adiponectin
HUABIO
catalog: HA723681B

domestic rabbit monoclonal (PSH14-83)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of mouse Adiponectin matched pair antibodies Capture: HA723679, Mouse Adiponectin Rabbit mAb [PSH14-82] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723679) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Adiponectin protein (HA210973) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723680, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of mouse Adiponectin matched pair antibodies Capture: HA723682, Mouse Adiponectin Rabbit mAb [PSH14-84] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723682) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Adiponectin protein (HA210973) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723680, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Adiponectin in mouse samples. Capture: HA723679, Mouse Adiponectin Rabbit mAb [PSH14-82] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Interpolated concentration of native Adiponectin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 32 ug/mL in mouse serum and 49 ng/ml in mouse lung cell supernatant. There was no detectable signal in NIH/3T3 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse Adiponectin
HUABIO
catalog: HA723682

domestic rabbit monoclonal (PSH14-84)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of mouse Adiponectin matched pair antibodies Capture: HA723682, Mouse Adiponectin Rabbit mAb [PSH14-84] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723682) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Adiponectin protein (HA210973) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA723680, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Adiponectin in mouse samples. Capture: HA723682, Mouse Adiponectin Rabbit mAb [PSH14-84] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] Interpolated concentration of native Adiponectin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 31 ug/mL in mouse serum and 47 ng/ml in mouse lung cell supernatant. There was no detectable signal in NIH/3T3 cell culture supernatant.

Interpolated concentrations of spiked Adiponectin in cell culture media samples. Capture: HA723682, Mouse Adiponectin Rabbit mAb [PSH14-84] Detector: HA723680, Mouse Adiponectin Rabbit mAb [PSH14-83] The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Adiponectin
HUABIO
catalog: HA751007

domestic rabbit monoclonal (PSH05-48)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (HA751007) at 1/2,000 dilution. Lane 1: Mouse white adipose tissue lysate Lane 2: Rat white adipose tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751007) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Adiponectin on human plasma lysates with Rabbit anti-Adiponectin antibody (HA751007) at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751007) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Adiponectin antibody (HA751007) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751007) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier