mouse monoclonal (10-E10)
reactivity: human, mouse, rat
application: flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: flow cytometry, immunohistochemistry - paraffin section
ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit monoclonal (PS00-61)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of SQSTM1 / p62 on different lysates with Rabbit anti-SQSTM1 / p62 antibody (HA721171) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721171) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SQSTM1 / p62 on different lysates with Rabbit anti-SQSTM1 / p62 antibody (HA721171) at 1/5,000 dilution. Lane 1: PANC-1 cell lysate (10 µg/Lane) Lane 2: RAW264.7 cell lysate (10 µg/Lane) Lane 3: Mouse liver tissue lysate (20 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721171) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SQSTM1 / p62 on different lysates with Rabbit anti-SQSTM1 / p62 antibody (HA721171) at 1/20,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si SQSTM1 / p62 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721171) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PS00-61)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of SQSTM1 / p62 on different lysates with Rabbit anti-SQSTM1 / p62 antibody (HA750510) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750510) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SQSTM1 / p62 on different lysates with Rabbit anti-SQSTM1 / p62 antibody (HA750510) at 1/5,000 dilution. Lane 1: PANC-1 cell lysate (10 µg/Lane) Lane 2: RAW264.7 cell lysate (10 µg/Lane) Lane 3: Mouse liver tissue lysate (20 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750510) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SQSTM1 / p62 on different lysates with Rabbit anti-SQSTM1 / p62 antibody (HA750510) at 1/20,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si SQSTM1 / p62 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750510) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis on Hela (1) and F9 (2) cell lysates using anti-SQSTM1 rabbit polyclonal antibodies.
ICC staining SQSTM1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue using anti- SQSTM1 rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier