PARK7/DJ1
HUABIO
catalog: ET1611-45
domestic rabbit monoclonal (SN07-21)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of PARK7/DJ1 on different lysates with Rabbit anti-PARK7/DJ1 antibody (ET1611-45) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: HepG2 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-45) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of PARK7/DJ1 on different lysates with Rabbit anti-PARK7/DJ1 antibody (ET1611-45) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si PARK7/DJ1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-45) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling PARK7/DJ1 with Rabbit anti-PARK7/DJ1 antibody (ET1611-45) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PARK7/DJ1 antibody (ET1611-45) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
Human PARK7
HUABIO
catalog: HA620001
mouse monoclonal (PSH15-67)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human PARK7 matched pair antibodies Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA620001) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PARK7 protein (HA211158) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA620002, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PARK7 in 0.125 mg/ml HL-60 lysate samples. Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] The concentrations of PARK7 were measured in duplicates, interpolated from the PARK7 standard curve and corrected for sample dilution. Undiluted samples are 0.125 mg/ml HL-60 lysate . The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PARK7 concentration was determined to be 64.8 ng/ml in HL-60 lysate.
quantity: 100μl
price: 649.00 USD
to the supplier
Human PARK7
HUABIO
catalog: HA620002
mouse monoclonal (PSH15-68)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human PARK7 matched pair antibodies Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA620001) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PARK7 protein (HA211158) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA620002, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PARK7 in 0.125 mg/ml HL-60 lysate samples. Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] The concentrations of PARK7 were measured in duplicates, interpolated from the PARK7 standard curve and corrected for sample dilution. Undiluted samples are 0.125 mg/ml HL-60 lysate . The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PARK7 concentration was determined to be 64.8 ng/ml in HL-60 lysate.
quantity: 100μl
price: 649.00 USD
to the supplier
Human PARK7
HUABIO
catalog: HA620002B
mouse monoclonal (PSH15-68)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human PARK7 matched pair antibodies Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA620001) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PARK7 protein (HA211158) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA620002, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PARK7 in 0.125 mg/ml HL-60 lysate samples. Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] The concentrations of PARK7 were measured in duplicates, interpolated from the PARK7 standard curve and corrected for sample dilution. Undiluted samples are 0.125 mg/ml HL-60 lysate . The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PARK7 concentration was determined to be 64.8 ng/ml in HL-60 lysate.
quantity: 100μl
price: 409.00 USD
to the supplier
Human PARK7
HUABIO
catalog: HA620002H
mouse monoclonal (PSH15-68)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human PARK7 matched pair antibodies Capture: HA620001 Human PARK7 Mouse mAb [PSH15-67] Detector: HA620002, Human PARK7 Mouse mAb [PSH15-68] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA620001) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PARK7 protein (HA211158) starting from 1,000,000 pg/ml to 0 pg/ml and detect antibody (HA620002, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 405.00 USD
to the supplier
Anti-PARK7/DJ1 Antibody [SN07-21]
HUABIO
catalog: ET1611-45TR
domestic rabbit monoclonal (SN07-21)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
quantity: 20 uL
price: 99 USD
to the supplier