YKL-40 / CHI3L1
HUABIO
catalog: EM1902-14-50UL
mouse monoclonal (10C1)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of YKL-40 / CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Application: IF-Tissue Species: Mouse Site: lung Sample: Paraffin-embedded section Antibody concentration: 1/200

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40 / CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1902-14) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier
YKL-40 / CHI3L1
HUABIO
catalog: EM1902-31
mouse monoclonal (A3G10)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of YKL-40 / CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40 / CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-31, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
YKL-40 / CHI3L1
HUABIO
catalog: HA600075
mouse monoclonal (A3G13)
reactivity: human
application: western blot

Western blot analysis of YKL-40 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600075, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
YKL-40 / CHI3L1
HUABIO
catalog: HA723824
domestic rabbit monoclonal (PSH16-26)
reactivity: human
application: western blot

Western blot analysis of YKL-40 / CHI3L1 on different lysates with Rabbit anti-YKL-40 / CHI3L1 antibody (HA723824) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 250 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723824) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 (positive) and 293T (negative) labeling YKL-40 / CHI3L1 with Rabbit anti-YKL-40 / CHI3L1 antibody (HA723824) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-YKL-40 / CHI3L1 antibody (HA723824) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
Human YKL-40
HUABIO
catalog: HA725088
domestic rabbit monoclonal (PSH13-08)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human YKL-40 matched pair antibodies Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725089, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native YKL-40 in U87 MG extract samples based on a 1000 µg/ml extract load. Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Interpolated concentration of native YKL-40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 15973 pg/mL in U-87 MG cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human YKL-40
HUABIO
catalog: HA725089
domestic rabbit monoclonal (PSH13-09)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human YKL-40 matched pair antibodies Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725089, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native YKL-40 in U87 MG extract samples based on a 1000 µg/ml extract load. Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Interpolated concentration of native YKL-40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 15973 pg/mL in U-87 MG cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human YKL-40
HUABIO
catalog: HA725090B
domestic rabbit monoclonal (PSH13-09)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human YKL-40 matched pair antibodies Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725089, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native YKL-40 in U87 MG extract samples based on a 1000 µg/ml extract load. Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Interpolated concentration of native YKL-40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 15973 pg/mL in U-87 MG cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier
Human YKL-40
HUABIO
catalog: HA725090H
domestic rabbit monoclonal (PSH13-09)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human YKL-40 matched pair antibodies Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08] Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 11,100 pg/ml to 0 pg/ml and detect antibody (HA725089, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
YKL-40 / CHI3L1
HUABIO
catalog: HA751594
domestic rabbit monoclonal (PSH16-26)
reactivity: human
application: western blot

Western blot analysis of YKL-40 / CHI3L1 on different lysates with Rabbit anti-YKL-40 / CHI3L1 antibody (HA751594) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 250 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751594) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 (positive) and 293T (negative) labeling YKL-40 / CHI3L1 with Rabbit anti-YKL-40 / CHI3L1 antibody (HA751594) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-YKL-40 / CHI3L1 antibody (HA751594) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
YKL-40 / CHI3L1
HUABIO
catalog: EM1902-14
mouse monoclonal (10C1)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
quantity: 100μl
price: 360.00 USD
to the supplier