CXCL13
HUABIO
catalog: ER65489
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of CXCL13 on different lysates with Rabbit anti-CXCL13 antibody (ER65489) at 1/2,000 dilution. Lane 1: 293T-OE-CXCL13 cell lysate Lane 2: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 13 kDa Observed band size: 13 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65489) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
CXCL13
HUABIO
catalog: HA722117
domestic rabbit monoclonal (PSH04-34)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CXCL13 on different lysates with Rabbit anti-CXCL13 antibody (HA722117) at 1/1,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-CXCL13 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 13 kDa Observed band size: 12/13 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722117) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa transfected with or without CXCL13 cells labeling CXCL13 with Rabbit anti-CXCL13 antibody (HA722117) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL13 antibody (HA722117) at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CXCL13 antibody (HA722117) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722117) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Human CXCL13
HUABIO
catalog: HA722791
domestic rabbit monoclonal (PSH07-05)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CXCL13 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722791) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human CXCL13 protein (HA211065) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722792, Biotin, 0.3 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human CXCL13 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722791) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human CXCL13 protein (HA211065) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722794, Biotin, 0.3 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CXCL13 in human THP-1 supernatant treated or untreated with Hu-IFN-r and LPS for 24 hours. Interpolated concentration of native CXCL13 was measured in duplicate at different sample concentrations and interpolated from the CXCL13 standard curves. Undiluted samples were 20% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 8,902 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CXCL13
HUABIO
catalog: HA722792
domestic rabbit monoclonal (PSH07-06)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CXCL13 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722791) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human CXCL13 protein (HA211065) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722792, Biotin, 0.3 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CXCL13 in human THP-1 supernatant treated or untreated with Hu-IFN-r and LPS for 24 hours. Interpolated concentration of native CXCL13 was measured in duplicate at different sample concentrations and interpolated from the CXCL13 standard curves. Undiluted samples were 20% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 8,902 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant.

Interpolated concentrations of spiked CXCL13 in human cell culture media samples. The concentrations of CXCL13 were measured in duplicates, interpolated from the CXCL13 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CXCL13
HUABIO
catalog: HA722793B
domestic rabbit monoclonal (PSH07-06)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human CXCL13 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722791) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human CXCL13 protein (HA211065) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722793B, 0.3 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CXCL13 in human THP-1 supernatant treated or untreated with Hu-IFN-r and LPS for 24 hours. Interpolated concentration of native CXCL13 was measured in duplicate at different sample concentrations and interpolated from the CXCL13 standard curves. Undiluted samples were 20% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 8,902 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant.

Interpolated concentrations of spiked CXCL13 in human cell culture media samples. The concentrations of CXCL13 were measured in duplicates, interpolated from the CXCL13 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human CXCL13
HUABIO
catalog: HA722794
domestic rabbit monoclonal (PSH07-07)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CXCL13 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722791) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human CXCL13 protein (HA211065) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722794, Biotin, 0.3 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CXCL13 in human THP-1 supernatant treated or untreated with Hu-IFN-r and LPS for 24 hours. Interpolated concentration of native CXCL13 was measured in duplicate at different sample concentrations and interpolated from the CXCL13 standard curves. Undiluted samples were 20% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 10,653 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant.

Interpolated concentrations of spiked CXCL13 in human cell culture media samples. The concentrations of CXCL13 were measured in duplicates, interpolated from the CXCL13 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human CXCL13
HUABIO
catalog: HA722795B
domestic rabbit monoclonal (PSH07-07)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human CXCL13 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722791) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human CXCL13 protein (HA211065) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722795B, 0.3 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CXCL13 in human THP-1 supernatant treated or untreated with Hu-IFN-r and LPS for 24 hours. Interpolated concentration of native CXCL13 was measured in duplicate at different sample concentrations and interpolated from the CXCL13 standard curves. Undiluted samples were 20% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 10,653 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant.

Interpolated concentrations of spiked CXCL13 in human cell culture media samples. The concentrations of CXCL13 were measured in duplicates, interpolated from the CXCL13 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
CXCL13
HUABIO
catalog: HA750943
domestic rabbit monoclonal (PSH04-34)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CXCL13 on different lysates with Rabbit anti-CXCL13 antibody (HA750943) at 1/1,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-CXCL13 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 13 kDa Observed band size: 12/13 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750943) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa transfected with or without CXCL13 cells labeling CXCL13 with Rabbit anti-CXCL13 antibody (HA750943) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL13 antibody (HA750943) at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CXCL13 antibody (HA750943) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750943) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier