MERTK
HUABIO
catalog: ET1602-21
domestic rabbit monoclonal (SR29-07)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MERTK on different lysates with Rabbit anti-MERTK antibody (ET1602-21) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 150-210 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MERTK on different lysates with Rabbit anti-MERTK antibody (ET1602-21) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MERTK KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 150-210 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-21) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MERTK antibody (ET1602-21) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Human MERTK
HUABIO
catalog: HA723479
domestic rabbit monoclonal (PSH12-69)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human MERTK matched pair antibodies Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723479) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MERTK protein (HA211172) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723480, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MERTK in HepG2 and MCF7 extract samples based on a 1000 µg/ml extract load. Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Interpolated concentration of native MERTK was measured in duplicate at different sample concentrations and interpolated from the MERTK standard curves. The mean MERTK concentration was determined to be 1,799 pg/mL in Hep G2 cell extract, There was no detectable signal in MCF7 cell extract.

Interpolated concentrations of native MERTK in human samples. Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Interpolated concentration of native MERTK was measured in duplicate at different sample concentrations. Undiluted samples were 10% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MERTK concentration was determined to be 119,371 pg/mL in human serum.
quantity: 100μl
price: 649.00 USD
to the supplier
Human MERTK
HUABIO
catalog: HA723480
domestic rabbit monoclonal (PSH12-70)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human MERTK matched pair antibodies Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723479) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MERTK protein (HA211172) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723480, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MERTK in HepG2 and MCF7 extract samples based on a 1000 µg/ml extract load. Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Interpolated concentration of native MERTK was measured in duplicate at different sample concentrations and interpolated from the MERTK standard curves. The mean MERTK concentration was determined to be 1,799 pg/mL in Hep G2 cell extract, There was no detectable signal in MCF7 cell extract.

Interpolated concentrations of native MERTK in human samples. Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Interpolated concentration of native MERTK was measured in duplicate at different sample concentrations. Undiluted samples were 10% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MERTK concentration was determined to be 119,371 pg/mL in human serum.
quantity: 100μl
price: 649.00 USD
to the supplier
Human MERTK
HUABIO
catalog: HA723481B
domestic rabbit monoclonal (PSH12-70)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human MERTK matched pair antibodies Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723479) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MERTK protein (HA211172) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723480, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MERTK in HepG2 and MCF7 extract samples based on a 1000 µg/ml extract load. Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Interpolated concentration of native MERTK was measured in duplicate at different sample concentrations and interpolated from the MERTK standard curves. The mean MERTK concentration was determined to be 1,799 pg/mL in Hep G2 cell extract, There was no detectable signal in MCF7 cell extract.

Interpolated concentrations of native MERTK in human samples. Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Interpolated concentration of native MERTK was measured in duplicate at different sample concentrations. Undiluted samples were 10% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MERTK concentration was determined to be 119,371 pg/mL in human serum.
quantity: 100μl
price: 409.00 USD
to the supplier
Human MERTK
HUABIO
catalog: HA723481H
domestic rabbit monoclonal (PSH12-70)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human MERTK matched pair antibodies Capture: HA723479, Human MERTK Rabbit mAb [PSH12-69] Detector: HA723480, Human MERTK Rabbit mAb [PSH12-70] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723479) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MERTK protein (HA211172) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA723480, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
MERTK
HUABIO
catalog: HA750036
domestic rabbit monoclonal (SR29-07)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MERTK on different lysates with Rabbit anti-MERTK antibody (HA750036) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 150-210 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750036) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MERTK on different lysates with Rabbit anti-MERTK antibody (HA750036) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MERTK KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 150-210 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750036) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MERTK antibody (HA750036) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750036) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier