MICA + MICB
HUABIO
catalog: ER1803-95
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (ER1803-95) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HL-60 cell lysate Lane 4: HUVEC cell lysate Lane 5: THP-1 cell lysate (negative) Lane 6: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-95) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
MICA
HUABIO
catalog: HA600002
mouse monoclonal (A4C5)
reactivity: human
application: western blot

Western blot analysis of MICA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600002, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Hela cell lysate
quantity: 100μl
price: 360.00 USD
to the supplier
MICA + MICB
HUABIO
catalog: HA723255
domestic rabbit monoclonal (PSH10-88)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (25 µg/Lane) Lane 3: THP-1 cell lysate (negative) (25 µg/Lane) Lane 4: NIH:OVCAR-3 cell lysate (25 µg/Lane) Lane 5: PC-3 cell lysate (low expression) (25 µg/Lane) Lane 6: U-2 OS cell lysate (25 µg/Lane) Lane 7: HUVEC cell lysate (25 µg/Lane) Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/2,000 dilution. Lane 1: Human MICA recombinant protein, 20ng/Lane Lane 2: Human MICB recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA + MICB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723255, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
Human MICA
HUABIO
catalog: HA723327
domestic rabbit monoclonal (PSH11-39)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human MICA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723327) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICA protein (HA210951) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723328, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MICA in Hela, K-562 and THP-1 extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native MICA was measured in duplicate at different sample concentrations and interpolated from the MICA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MICA concentration was determined to be 7,949 pg/mL in Hela and 1,379 pg/mL in K-562 cell extract. There was no detectable signal in THP-1 cell extract.

Interpolated concentrations of spiked MICA in cell culture media samples. The concentrations of MICA were measured in duplicates, interpolated from the MICA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human MICA
HUABIO
catalog: HA723328
domestic rabbit monoclonal (PSH11-40)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human MICA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723327) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICA protein (HA210951) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723328, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MICA in Hela, K-562 and THP-1 extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native MICA was measured in duplicate at different sample concentrations and interpolated from the MICA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MICA concentration was determined to be 7,949 pg/mL in Hela and 1,379 pg/mL in K-562 cell extract. There was no detectable signal in THP-1 cell extract.

Interpolated concentrations of spiked MICA in cell culture media samples. The concentrations of MICA were measured in duplicates, interpolated from the MICA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human MICA
HUABIO
catalog: HA723329B
domestic rabbit monoclonal (PSH11-40)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human MICA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723327) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICA protein (HA210951) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723329B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MICA in Hela, K-562 and THP-1 extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native MICA was measured in duplicate at different sample concentrations and interpolated from the MICA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MICA concentration was determined to be 7,949 pg/mL in Hela and 1,379 pg/mL in K-562 cell extract. There was no detectable signal in THP-1 cell extract.

Interpolated concentrations of spiked MICA in cell culture media samples. The concentrations of MICA were measured in duplicates, interpolated from the MICA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
MICA
HUABIO
catalog: HA723549
domestic rabbit monoclonal (PSH13-60)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of MICA on different lysates with Rabbit anti-MICA antibody (HA723549) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (25 µg/Lane) Lane 3: THP-1 cell lysate (negative) (25 µg/Lane) Lane 4: OVCAR-3 cell lysate (25 µg/Lane) Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723549) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723549, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

MICA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723549 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723549 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723549 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723549 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier
MICA + MICB
HUABIO
catalog: HA751380
domestic rabbit monoclonal (PSH10-88)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA751380) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (25 µg/Lane) Lane 3: THP-1 cell lysate (negative) (25 µg/Lane) Lane 4: NIH:OVCAR-3 cell lysate (25 µg/Lane) Lane 5: PC-3 cell lysate (low expression) (25 µg/Lane) Lane 6: U-2 OS cell lysate (25 µg/Lane) Lane 7: HUVEC cell lysate (25 µg/Lane) Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751380) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA751380) at 1/2,000 dilution. Lane 1: Human MICA recombinant protein, 20ng/Lane Lane 2: Human MICB recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751380) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA + MICB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751380, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier
MICA
HUABIO
catalog: HA751479
domestic rabbit monoclonal (PSH13-60)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of MICA on different lysates with Rabbit anti-MICA antibody (HA751479) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (25 µg/Lane) Lane 3: THP-1 cell lysate (negative) (25 µg/Lane) Lane 4: OVCAR-3 cell lysate (25 µg/Lane) Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751479) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751479, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

MICA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751479 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751479 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751479 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751479 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier