Pan-Cadherin
HUABIO
catalog: ET1609-70-50UL
domestic rabbit monoclonal (ST54-01)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Pan-Cadherin on different lysates with Rabbit anti-Pan-Cadherin antibody (ET1609-70) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: Mouse embryo tissue lysate (40 µg/Lane) Lane 4: Mouse placenta tissue lysate (40 µg/Lane) Lane 5: Rat embryo tissue lysate (40 µg/Lane) Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-70) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Pan-Cadherin on different lysates with Rabbit anti-Pan-Cadherin antibody (ET1609-70) at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-70) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling Pan-Cadherin with Rabbit anti-Pan-Cadherin antibody (ET1609-70) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pan-Cadherin antibody (ET1609-70) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 50μl
price: 205.00 USD
to the supplier
Human VE Cadherin
HUABIO
catalog: HA721838
domestic rabbit monoclonal (PSH02-61)
reactivity: human
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
Human VE Cadherin
HUABIO
catalog: HA721839
domestic rabbit monoclonal (PSH02-62)
reactivity: human
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
VE Cadherin
HUABIO
catalog: HA721943
domestic rabbit monoclonal (PSH03-15)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of VE Cadherin on different lysates with Rabbit anti-VE Cadherin antibody (HA721943) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721943) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of VE Cadherin on different lysates with Rabbit anti-VE Cadherin antibody (HA721943) at 1/2,000 dilution. Lane 1: Human lung tissue lysate Lane 2: Human kidney tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 88 kDa Observed band size: 120-140 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721943) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of EA.hy926 cells labeling VE Cadherin with Rabbit anti-VE Cadherin antibody (HA721943) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VE Cadherin antibody (HA721943) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
Human VE Cadherin
HUABIO
catalog: HA722635B
domestic rabbit monoclonal (PSH02-62)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier
Pan-Cadherin
HUABIO
catalog: HA750190
domestic rabbit monoclonal (ST54-01)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Pan-Cadherin on different lysates with Rabbit anti-Pan-Cadherin antibody (HA750190) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: Mouse embryo tissue lysate (40 µg/Lane) Lane 4: Mouse placenta tissue lysate (40 µg/Lane) Lane 5: Rat embryo tissue lysate (40 µg/Lane) Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750190) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Pan-Cadherin on different lysates with Rabbit anti-Pan-Cadherin antibody (HA750190) at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750190) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling Pan-Cadherin with Rabbit anti-Pan-Cadherin antibody (HA750190) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pan-Cadherin antibody (HA750190) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
VE Cadherin
HUABIO
catalog: HA750856
domestic rabbit monoclonal (PSH03-15)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of VE Cadherin on different lysates with Rabbit anti-VE Cadherin antibody (HA750856) at 1/2,000 dilution. Lane 1: EA.hy926-si NT cell lysate Lane 2: EA.hy926-si VE Cadherin cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 140 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750856) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of VE Cadherin on different lysates with Rabbit anti-VE Cadherin antibody (HA750856) at 1/2,000 dilution. Lane 1: Human lung tissue lysate Lane 2: Human kidney tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 88 kDa Observed band size: 120-140 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750856) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of EA.hy926 cells labeling VE Cadherin with Rabbit anti-VE Cadherin antibody (HA750856) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VE Cadherin antibody (HA750856) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
Pan-Cadherin
HUABIO
catalog: IRS285MS
domestic rabbit polyclonal
reactivity: human

mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-Pan-Cadherin antibody (IRS285MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 279.00 USD
to the supplier
Pan-Cadherin
HUABIO
catalog: IRS287RT
domestic rabbit polyclonal
reactivity: human

mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rat anti-Pan-Cadherin antibody (IRS287RT) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900810). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 279.00 USD
to the supplier