GST | HUABIO 1003-8 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GST

catalog :

1003-8

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, ELISA, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

GST was immunoprecipitated in 0.2mg 293T-OE-GST cell lysate with 1003-8 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using 1003-8 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input) Lane 2: Rabbit IgG instead of 1003-8 in 293T transfected with GST-tagged Histone H3.1 cell lysate Lane 3: 1003-8 IP in 293T transfected with GST-tagged Histone H3.1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds

image 2 :

Western blot analysis on GST fusion protein (26kDa) using anti-GST polyclonal antibody.

image 3 :

Immunocytochemistry analysis of 293T cells transfected with or without GST labeling GST with Rabbit anti-GST antibody (1003-8) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody (1003-8) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

1003-8

Target name :

GST

Species reactivity :

Species independent

Applications :

WB,ELISA,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within GST protein.

Uniprot id :

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

GST fusion-protein

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:10,000-1:100,000 ;ELISA: 1:10,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage