TMEM132A | HUABIO 0903-8 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TMEM132A

catalog :

0903-8

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TMEM132A on different lysates with Rabbit anti-TMEM132A antibody (0903-8) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 55 kDa Observed band size: 140 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0903-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of TMEM132A on different lysates with Rabbit anti-TMEM132A antibody (0903-8) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 110/44/55 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0903-8) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining Transmembrane protein 132A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

0903-8

Target name :

TMEM132A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human TMEM132A aa 204-253 / 1,023.

Uniprot id :

Q24JP5>SwissProt: Q24JP5 Human;SwissProt: Q922P8 Mouse;SwissProt: Q80WF4 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse brain tissue lysate, rat brain tissue lysate, MCF-7 cell lysate, Hela, SHG-44, mouse brain tissue.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Endoplasmic reticulum. Golgi apparatus. Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/0903-8_4.jpg

Pic legend4 :

ICC staining Transmembrane protein 132A in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/0903-8_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Transmembrane protein 132A antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/0903-8_6.jpg

Pic legend6 :

Flow cytometric analysis of SH-SY-5Y cells with Transmembrane protein 132A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage