Carcino Embryonic Antigen CEA | HUABIO 0807-10 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Carcino Embryonic Antigen CEA

catalog :

0807-10

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Carcino Embryonic Antigen CEA on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining Carcino Embryonic Antigen CEA in SK-Br-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carcino Embryonic Antigen CEA polyclonal antibody at a dilution of 1/200 for at least 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining Carcino Embryonic Antigen CEA in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carcino Embryonic Antigen CEA polyclonal antibody at a dilution of 1/200 for at least 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

0807-10

Target name :

Carcino Embryonic Antigen CEA

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CEA aa 153-216.

Uniprot id :

P06731>SwissProt: P06731 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

MCF-7 cell lysate, SK-Br-3, SW620, human liver cancer tissue, human colon cancer tissue, HepG2.

Molecular wt :

Predicted band size: 77 kDa

Subcellular location :

Cell membrane, Membrane.

Concentration :

1 mg/ml.

Recommended dilutions :

WB:1:1,000-1:2,000;IF-Cell:1:50-1:200;IHC-P:1:50-1:200;FC:1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/0807-10_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Carcino Embryonic Antigen CEA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0807-10) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/0807-10_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Carcino Embryonic Antigen CEA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0807-10) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/0807-10_6.jpg

Pic legend6 :

Flow cytometric analysis of Carcino Embryonic Antigen CEA was done on HepG2 cells. The cells were fixed, permeabilized and stained with Carcino Embryonic Antigen CEA antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

more info or order :

HUABIO product webpage