Junctophilin-2 | HUABIO 0407-9 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Junctophilin-2

catalog :

0407-9

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Junctophilin-2 on different lysates with Rabbit anti-Junctophilin-2 antibody (0407-9) at 1/2,000 dilution. Lane 1: Rat skeletal muscle tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0407-9) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Junctophilin-2 antibody (0407-9) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-9) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Junctophilin-2 antibody (0407-9) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-9) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

0407-9

Target name :

Junctophilin-2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Junctophilin-2 aa 418-467 / 696.

Uniprot id :

Q9BR39>SwissProt: Q9BR39 Human;SwissProt: Q9ET78 Mouse;SwissProt: Q2PS20 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Rat skeletal muscle tissue lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue, rat heart tissue, human striated muscle tissue, human heart tissue, mouse skeletal muscle tissue, mouse heart tissue, L6, C2C12.

Molecular wt :

Predicted band size: 74 kDa

Subcellular location :

Cell membrane, Sarcoplasmic reticulum membrane, Endoplasmic reticulum membrane; Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:100-1:400 ;FC: 1:50-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/0407-9_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Junctophilin-2 antibody (0407-9) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-9) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/0407-9_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Junctophilin-2 antibody (0407-9) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-9) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/0407-9_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Junctophilin-2 antibody (0407-9) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-9) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/0407-9_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Junctophilin-2 antibody (0407-9) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0407-9) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/0407-9_8.jpg

Pic legend8 :

Flow cytometric analysis of L6 cells labeling Junctophilin-2. Cells were fixed and permeabilized. Then stained with the primary antibody (0407-9, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/0407-9_9.jpg

Pic legend9 :

Flow cytometric analysis of C2C12 cells labeling Junctophilin-2. Cells were fixed and permeabilized. Then stained with the primary antibody (0407-9, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage