LC3B antibody | GeneTex GTX82986 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

LC3B antibody

catalog :

GTX82986

quantity :

100.00μl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, FACS, immunohistochemistry - frozen section

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:2000; fig 2	Sukseree S, Chen Y, Laggner M, Gruber F, Petit V, Nagelreiter I, et al. Tyrosinase-Cre-Mediated Deletion of the Autophagy Gene Atg7 Leads to Accumulation of the RPE65 Variant M450 in the Retinal Pigment Epithelium of C57BL/6 Mice. PLoS ONE. 2016;11:e0161640 pubmed publisher
western blot; human; fig 4	Colangelo T, Polcaro G, Ziccardi P, Muccillo L, Galgani M, Pucci B, et al. The miR-27a-calreticulin axis affects drug-induced immunogenic cell death in human colorectal cancer cells. Cell Death Dis. 2016;7:e2108 pubmed publisher

product information

Catalog No :

GTX82986

Product Name :

LC3B antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Product Type :

Primary antibody

Size :

100.00μl

list of Pubmed id :

21970855,22262369,20159967,23340736,23669018

Product Description :

Rabbit polyclonal antibody to LC3B

CrossReactivity :

Hu, Ms

Background :

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

Immunogen :

Synthetic peptide - N-terminal region of the human LC3, isoform B protein

Specificity :

B isoform of LC3

Buffer :

Tris-glycine, 150mM NaCl containing 0.05% sodium azide

Uses :

FACS, ICC/IF, IHC, IHC-Fr, WB

Concentration :

1.47mg/ml

General References :

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles

Image Legend_4 :

Immunohistochemistry: LC3B Antibody [GTX82986] - LC3B staining in treated U87-MG cultured & subcutaneous tumors.

Image Legend_5 :

Immunohistochemistry: LC3B Antibody [GTX82986] - LC3B staining in gliobastoma multiform tissue.

Image Legend_6 :

Immunohistochemistry: LC3B Antibody [GTX82986] - Staining of treated U373-MG (human glioblastoma.