product summary
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company name :
GeneTex
product type :
antibody
product name :
HEC1 antibody [9G3.23]
catalog :
GTX70268
quantity :
100.00μg
price :
299 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
9G3.23
reactivity :
Coturnix, human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
more info or order :
citations: 53
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 0.5 ug/ml; loading ...; fig 4a
Malaby H, Dumas M, Ohi R, Stumpff J. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. J Cell Biol. 2019;218:1218-1234 pubmed publisher
  • western blot; human; loading ...; fig 1f
Fang Y, Kong Y, Xi J, Zhu M, Zhu T, Jiang T, et al. Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity. Oncotarget. 2016;7:79327-79341 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2g
Chung H, Park J, Lee N, Kim H, Jang C. Phosphorylation of Astrin Regulates Its Kinetochore Function. J Biol Chem. 2016;291:17579-92 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 3d
  • western blot; human; 1:1000; fig 3e
O Connor A, Maffini S, Rainey M, Kaczmarczyk A, Gaboriau D, Musacchio A, et al. Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint. Biol Open. 2015;5:11-9 pubmed publisher
  • immunocytochemistry; human
Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. 2015;290:27053-66 pubmed publisher
  • western blot; Coturnix
Miller S, Johnson M, Stukenberg P. Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1). Curr Biol. 2008;18:1785-91 pubmed publisher
Alfonso Pérez T, Hayward D, Holder J, Gruneberg U, Barr F. MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling. J Cell Biol. 2019;218:1108-1117 pubmed publisher
Janssen L, Averink T, Blomen V, Brummelkamp T, Medema R, Raaijmakers J. Loss of Kif18A Results in Spindle Assembly Checkpoint Activation at Microtubule-Attached Kinetochores. Curr Biol. 2018;28:2685-2696.e4 pubmed publisher
Petsalaki E, Dandoulaki M, Zachos G. The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling. J Cell Biol. 2018;217:861-876 pubmed publisher
Ferrara M, Sessa G, Fiore M, Bernard F, Asteriti I, Cundari E, et al. Small molecules targeted to the microtubule-Hec1 interaction inhibit cancer cell growth through microtubule stabilization. Oncogene. 2018;37:231-240 pubmed publisher
Shirnekhi H, Kelley E, DeLuca J, Herman J. Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation. Mol Biol Cell. 2017;28:2035-2041 pubmed publisher
Janczyk P, Skorupka K, Tooley J, Matson D, Kestner C, WEST T, et al. Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores. Dev Cell. 2017;41:438-449.e4 pubmed publisher
Huis In t Veld P, Jeganathan S, Petrovic A, Singh P, John J, Krenn V, et al. Molecular basis of outer kinetochore assembly on CENP-T. elife. 2016;5: pubmed publisher
Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova Y, et al. Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores. Cell. 2016;167:1028-1040.e15 pubmed publisher
Redli P, Gasic I, Meraldi P, Nigg E, Santamaria A. The Ska complex promotes Aurora B activity to ensure chromosome biorientation. J Cell Biol. 2016;215:77-93 pubmed
Zhou H, Wang T, Zheng T, Teng J, Chen J. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 2016;7:10151 pubmed publisher
Tauchman E, Boehm F, DeLuca J. Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Commun. 2015;6:10036 pubmed publisher
Espert A, Uluocak P, Bastos R, Mangat D, Graab P, Gruneberg U. PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing. J Cell Biol. 2014;206:833-42 pubmed publisher
Häfner J, Mayr M, Möckel M, Mayer T. Pre-anaphase chromosome oscillations are regulated by the antagonistic activities of Cdk1 and PP1 on Kif18A. Nat Commun. 2014;5:4397 pubmed publisher
Huang L, Chang C, Lee Y, Huang J, Chuang S, Chang J, et al. Inhibition of Hec1 as a novel approach for treatment of primary liver cancer. Cancer Chemother Pharmacol. 2014;74:511-20 pubmed publisher
Basilico F, Maffini S, Weir J, Prumbaum D, Rojas A, Zimniak T, et al. The pseudo GTPase CENP-M drives human kinetochore assembly. elife. 2014;3:e02978 pubmed publisher
Matson D, Stukenberg P. CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status. J Cell Biol. 2014;205:541-54 pubmed publisher
Petsalaki E, Zachos G. Chk2 prevents mitotic exit when the majority of kinetochores are unattached. J Cell Biol. 2014;205:339-56 pubmed publisher
Huang L, Chang C, Lee Y, Chang J, Huang J, Chuang S, et al. Activity of a novel Hec1-targeted anticancer compound against breast cancer cell lines in vitro and in vivo. Mol Cancer Ther. 2014;13:1419-30 pubmed publisher
Caldas G, Deluca K, DeLuca J. KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity. J Cell Biol. 2013;203:957-69 pubmed
Beck J, Maerki S, Posch M, Metzger T, Persaud A, Scheel H, et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol. 2013;15:430-9 pubmed publisher
Buheitel J, Stemmann O. Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate. EMBO J. 2013;32:666-76 pubmed publisher
Lawo S, Hasegan M, Gupta G, Pelletier L. Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Nat Cell Biol. 2012;14:1148-58 pubmed publisher
De Antoni A, Maffini S, Knapp S, Musacchio A, Santaguida S. A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B. J Cell Biol. 2012;199:269-84 pubmed publisher
Matson D, Demirel P, Stukenberg P, Burke D. A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. Genes Dev. 2012;26:542-7 pubmed publisher
Chan Y, Jeyaprakash A, Nigg E, Santamaria A. Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction. J Cell Biol. 2012;196:563-71 pubmed publisher
Krenn V, Wehenkel A, Li X, Santaguida S, Musacchio A. Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction. J Cell Biol. 2012;196:451-67 pubmed publisher
Serio G, Margaria V, Jensen S, Oldani A, Bartek J, Bussolino F, et al. Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores. Proc Natl Acad Sci U S A. 2011;108:17337-42 pubmed publisher
Barnhart M, Kuich P, Stellfox M, Ward J, Bassett E, Black B, et al. HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore. J Cell Biol. 2011;194:229-43 pubmed publisher
Santaguida S, Vernieri C, Villa F, Ciliberto A, Musacchio A. Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction. EMBO J. 2011;30:1508-19 pubmed publisher
Screpanti E, De Antoni A, Alushin G, Petrovic A, Melis T, Nogales E, et al. Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore. Curr Biol. 2011;21:391-8 pubmed publisher
Tooley J, Miller S, Stukenberg P. The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement. Mol Biol Cell. 2011;22:1217-26 pubmed publisher
Sundin L, Guimaraes G, DeLuca J. The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis. Mol Biol Cell. 2011;22:759-68 pubmed publisher
Zanet J, Freije A, Ruiz M, Coulon V, Sanz J, Chiesa J, et al. A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication. PLoS ONE. 2010;5:e15701 pubmed publisher
Zeng K, Bastos R, Barr F, Gruneberg U. Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2. J Cell Biol. 2010;191:1315-32 pubmed publisher
Petrovic A, Pasqualato S, Dube P, Krenn V, Santaguida S, Cittaro D, et al. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. J Cell Biol. 2010;190:835-52 pubmed publisher
Screpanti E, Santaguida S, Nguyen T, Silvestri R, Gussio R, Musacchio A, et al. A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds. PLoS ONE. 2010;5:e11603 pubmed publisher
Santaguida S, Tighe A, D Alise A, Taylor S, Musacchio A. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol. 2010;190:73-87 pubmed publisher
Wu G, Wei R, Cheng E, Ngo B, Lee W. Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1. Mol Biol Cell. 2009;20:4686-95 pubmed publisher
Chan Y, Fava L, Uldschmid A, Schmitz M, Gerlich D, Nigg E, et al. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J Cell Biol. 2009;185:859-74 pubmed publisher
Zhu H, Coppinger J, Jang C, Yates J, Fang G. FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle. J Cell Biol. 2008;183:835-48 pubmed publisher
Wu G, Qiu X, Zhou L, Zhu J, Chamberlin R, Lau J, et al. Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal. Cancer Res. 2008;68:8393-9 pubmed publisher
Barr A, Gergely F. MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics. Mol Cell Biol. 2008;28:7199-211 pubmed publisher
Jang C, Wong J, Coppinger J, Seki A, Yates J, Fang G. DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement. J Cell Biol. 2008;181:255-67 pubmed publisher
Wong J, Lerrigo R, Jang C, Fang G. Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain. Mol Biol Cell. 2008;19:2083-91 pubmed publisher
Montembault E, Dutertre S, Prigent C, Giet R. PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores. J Cell Biol. 2007;179:601-9 pubmed
Wong J, Fang G. HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture. J Cell Biol. 2006;173:879-91 pubmed
Klein U, Nigg E, Gruneberg U. Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP. Mol Biol Cell. 2006;17:2547-58 pubmed
product information
Catalog No :
GTX70268
Product Name :
HEC1 antibody [9G3.23]
Host Species :
Mouse
Clonality :
Monoclonal
Product Type :
Primary antibody
Size :
100.00μg
List Price :
299 USD
list of Pubmed id :
24344188,21407176,19029337,22331848,20657644,23071153,21187932,18321990,21987812,21270439,19026542,20624901,21768289,22371557,18411309,22426531,21325630,20819937,21353556,16571674,17998396,23361318,21187329,16769820,23086237,18809577,19468067,18922912,19776357,23455478,24694948,24798733,24862574,25006165,25038613,25048371,25246613
Clone :
9G3.23
Isotype :
IgG2a
Product Description :
Mouse monoclonal antibody to human HEC1
CrossReactivity :
Hu, Ms, Pig, Hm, AGMK
Background :
Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation.
Immunogen :
Human HEC1 protein consisting of amino acids 56-642.
Buffer :
Phosphate-buffered saline, pH 7.4, containing no preservatives
Uses :
FACS, ICC/IF, IHC, IP, WB, WB-Ag
Concentration :
1mg/ml
General References :
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Image Legend_4 :
1/1000 dilution staining ~80 kDa HEC 1 in Hela cell lysate (30mg per well) by Western blot.
more info or order :
company information
GeneTex
2456 Alton Pkwy
Irvine, CA92606
info@genetex.com
http://www.genetex.com
1-877-436-3839
headquarters: USA