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company name :
GeneTex
product type :
antibody
product name :
Rad51 antibody [14B4]
catalog :
GTX70230
quantity :
100.00μg
price :
299 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
14B4
reactivity :
human, mouse, chicken
application :
western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:500; loading ...; fig 4a
Tan S, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017;169:1105-1118.e15 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 3
Zhang H, Liu H, Chen Y, Yang X, Wang P, Liu T, et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016;7:10201 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 3B
Uringa E, Baldeyron C, Odijk H, Wassenaar E, van Cappellen W, Maas A, et al. A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity. DNA Repair (Amst). 2015;25:27-40 pubmed publisher
Perl A, O Connor C, Fa P, Mayca Pozo F, Zhang J, Zhang Y, et al. Protein phosphatase 2A controls ongoing DNA replication by binding to and regulating cell division cycle 45 (CDC45). J Biol Chem. 2019;294:17043-17059 pubmed publisher
Heijink A, Everts M, Honeywell M, Richards R, Kok Y, de Vries E, et al. Modeling of Cisplatin-Induced Signaling Dynamics in Triple-Negative Breast Cancer Cells Reveals Mediators of Sensitivity. Cell Rep. 2019;28:2345-2357.e5 pubmed publisher
Heijink A, Talens F, Jae L, van Gijn S, Fehrmann R, Brummelkamp T, et al. BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity. Nat Commun. 2019;10:100 pubmed publisher
van Gijn S, Wierenga E, van den Tempel N, Kok Y, Heijink A, Spierings D, et al. TPX2/Aurora kinase A signaling as a potential therapeutic target in genomically unstable cancer cells. Oncogene. 2019;38:852-867 pubmed publisher
Mansour W, Tennstedt P, Volquardsen J, Oing C, Kluth M, Hube Magg C, et al. Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer. Sci Rep. 2018;8:3947 pubmed publisher
Browning C, Wise J. Prolonged exposure to particulate chromate inhibits RAD51 nuclear import mediator proteins. Toxicol Appl Pharmacol. 2017;331:101-107 pubmed publisher
Jiang Y, Verbiest T, Devery A, Bokobza S, Weber A, Leszczynska K, et al. Hypoxia Potentiates the Radiation-Sensitizing Effect of Olaparib in Human Non-Small Cell Lung Cancer Xenografts by Contextual Synthetic Lethality. Int J Radiat Oncol Biol Phys. 2016;95:772-81 pubmed publisher
Zhu J, Chen H, Guo X, Qiu X, Hu C, Chamberlin A, et al. Synthesis, molecular modeling, and biological evaluation of novel RAD51 inhibitors. Eur J Med Chem. 2015;96:196-208 pubmed publisher
Waddell N, Pajic M, Patch A, Chang D, Kassahn K, Bailey P, et al. Whole genomes redefine the mutational landscape of pancreatic cancer. Nature. 2015;518:495-501 pubmed publisher
White M, Kaminski R, Khalili K, Wollebo H. Rad51 activates polyomavirus JC early transcription. PLoS ONE. 2014;9:e110122 pubmed publisher
Pefani D, Latusek R, Pires I, Grawenda A, Yee K, Hamilton G, et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat Cell Biol. 2014;16:962-71, 1-8 pubmed publisher
Karnak D, Engelke C, Parsels L, Kausar T, Wei D, Robertson J, et al. Combined inhibition of Wee1 and PARP1/2 for radiosensitization in pancreatic cancer. Clin Cancer Res. 2014;20:5085-96 pubmed publisher
Naipal K, Verkaik N, Ameziane N, van Deurzen C, Ter Brugge P, Meijers M, et al. Functional ex vivo assay to select homologous recombination-deficient breast tumors for PARP inhibitor treatment. Clin Cancer Res. 2014;20:4816-26 pubmed publisher
Kaminski R, Wollebo H, Datta P, White M, Amini S, Khalili K. Interplay of Rad51 with NF-?B pathway stimulates expression of HIV-1. PLoS ONE. 2014;9:e98304 pubmed publisher
Pfäffle H, Wang M, Gheorghiu L, Ferraiolo N, Greninger P, Borgmann K, et al. EGFR-activating mutations correlate with a Fanconi anemia-like cellular phenotype that includes PARP inhibitor sensitivity. Cancer Res. 2013;73:6254-63 pubmed publisher
Engelke C, Parsels L, Qian Y, Zhang Q, Karnak D, Robertson J, et al. Sensitization of pancreatic cancer to chemoradiation by the Chk1 inhibitor MK8776. Clin Cancer Res. 2013;19:4412-21 pubmed publisher
Wei D, Parsels L, Karnak D, Davis M, Parsels J, Marsh A, et al. Inhibition of protein phosphatase 2A radiosensitizes pancreatic cancers by modulating CDC25C/CDK1 and homologous recombination repair. Clin Cancer Res. 2013;19:4422-32 pubmed publisher
Zhu J, Zhou L, Wu G, Konig H, Lin X, Li G, et al. A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia. EMBO Mol Med. 2013;5:353-65 pubmed publisher
Oplustilova L, Wolanin K, Mistrik M, Korinkova G, Simkova D, Bouchal J, et al. Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment. Cell Cycle. 2012;11:3837-50 pubmed publisher
Borst G, McLaughlin M, Kyula J, Neijenhuis S, Khan A, Good J, et al. Targeted radiosensitization by the Chk1 inhibitor SAR-020106. Int J Radiat Oncol Biol Phys. 2013;85:1110-8 pubmed publisher
Sharma S, Hicks J, Chute C, Brennan J, Ahn J, Glover T, et al. REV1 and polymerase ? facilitate homologous recombination repair. Nucleic Acids Res. 2012;40:682-91 pubmed publisher
Lee M, Daniels M, Garnett M, Venkitaraman A. A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor. Oncogene. 2011;30:3360-9 pubmed publisher
Klammer H, KADHIM M, Iliakis G. Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells. Cancer Res. 2010;70:8498-506 pubmed publisher
Ammazzalorso F, Pirzio L, Bignami M, Franchitto A, Pichierri P. ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery. EMBO J. 2010;29:3156-69 pubmed publisher
Graeser M, McCarthy A, Lord C, Savage K, Hills M, Salter J, et al. A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer. Clin Cancer Res. 2010;16:6159-68 pubmed publisher
Bowen C, Gelmann E. NKX3.1 activates cellular response to DNA damage. Cancer Res. 2010;70:3089-97 pubmed publisher
Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S. A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. Nucleic Acids Res. 2010;38:e129 pubmed publisher
Dungey F, Caldecott K, Chalmers A. Enhanced radiosensitization of human glioma cells by combining inhibition of poly(ADP-ribose) polymerase with inhibition of heat shock protein 90. Mol Cancer Ther. 2009;8:2243-54 pubmed publisher
Carlessi L, De Filippis L, Lecis D, Vescovi A, Delia D. DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression. Cell Death Differ. 2009;16:795-806 pubmed publisher
Warren M, Lord C, Masabanda J, Griffin D, Ashworth A. Phenotypic effects of heterozygosity for a BRCA2 mutation. Hum Mol Genet. 2003;12:2645-56 pubmed
Kao G, McKenna W, Guenther M, Muschel R, Lazar M, Yen T. Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response. J Cell Biol. 2003;160:1017-27 pubmed
Masson J, Davies A, Hajibagheri N, Van Dyck E, Benson F, Stasiak A, et al. The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51. EMBO J. 1999;18:6552-60 pubmed
product information
Catalog No :
GTX70230
Product Name :
Rad51 antibody [14B4]
Host Species :
Mouse
Clonality :
Monoclonal
Product Type :
Primary antibody
Size :
100.00μg
List Price :
299 USD
list of Pubmed id :
22983061,12928478,10562567,21926160,20802463,19229246,12668657,20802015,20861183,20385602,20395202,19671736,21399666,23341130,23780887,23804422,23966292,22981708,24847939,24963051,25117293,25218637,25310191,25463395,25719666,25874343
Clone :
14B4
Isotype :
IgG2b
Product Description :
Mouse monoclonal antibody to human Rad51
CrossReactivity :
Hu, Ms, Chk
Background :
Rad51, a 37 kDa protein, is the human homologue of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).
Immunogen :
Full length (amino acids 1-338) Rad51 expressed in E. coli.
Buffer :
Phosphate-buffered saline, pH 7.4, containing no preservatives
Uses :
ICC/IF, IHC-P, IP, WB
Concentration :
1.27mg/ml
General References :
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Image Legend_4 :
Mouse anti-RAD51 14B4 antibody (GTX70230) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody (GTX100469) was used for subsequent WB detection of immunoprecipated RAD51. Whole cell extract was used as input.
Image Legend_5 :
Rad51 antibody [14B4] detects Rad51 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at a dilution of 1:500 and developed with Trident Sharp-ECL (GTX14698).
more info or order :
company information
GeneTex
2456 Alton Pkwy
Irvine, CA92606
info@genetex.com
http://www.genetex.com
1-877-436-3839
headquarters: USA