product summary
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company name :
GeneTex
product type :
antibody
product name :
Rad50 antibody [13B3]
catalog :
GTX70228
quantity :
100.00μg
price :
299 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
13B3
reactivity :
human, mouse, rat, Escherichia coli
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
more info or order :
GeneTex product webpage
citations: 95
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 1f
Lee J, Mand M, Kao C, Zhou Y, Ryu S, Richards A, et al. ATM directs DNA damage responses and proteostasis via genetically separable pathways. Sci Signal. 2018;11: pubmed publisher
  • western blot; Escherichia coli; fig 6a
Deshpande R, Lee J, Paull T. Rad50 ATPase activity is regulated by DNA ends and requires coordination of both active sites. Nucleic Acids Res. 2017;45:5255-5268 pubmed publisher
  • immunoprecipitation; human; fig 3
  • immunocytochemistry; human; 1:200; fig 3
  • western blot; human; 1:1000; fig 3
Lee K, Im J, Shibata E, Park J, Handa N, Kowalczykowski S, et al. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. Nat Commun. 2015;6:7744 pubmed publisher
  • western blot; human; fig 5
Harten S, Oey H, Bourke L, Bharti V, Isbel L, Daxinger L, et al. The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers. BMC Biol. 2015;13:21 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; mouse
Xiong J, Todorova D, Su N, Kim J, Lee P, Shen Z, et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. J Cell Biol. 2015;208:513-20 pubmed publisher
  • western blot; human
Anderson V, Walton M, Eve P, Boxall K, Antoni L, Caldwell J, et al. CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res. 2011;71:463-72 pubmed publisher
Ly P, Brunner S, Shoshani O, Kim D, Lan W, Pyntikova T, et al. Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements. Nat Genet. 2019;51:705-715 pubmed publisher
Li Z, Li J, Kong Y, Yan S, Ahmad N, Liu X. Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response. Cancer Res. 2017;77:3169-3180 pubmed publisher
Mariggiò G, Koch S, Zhang G, Weidner Glunde M, Rückert J, Kati S, et al. Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency. PLoS Pathog. 2017;13:e1006335 pubmed publisher
Staples C, Barone G, Myers K, Ganesh A, Gibbs Seymour I, Patil A, et al. MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response. Cell Rep. 2016;16:2565-2575 pubmed publisher
Petroni M, Sardina F, Heil C, Sahún Roncero M, Colicchia V, Veschi V, et al. The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress. Cell Death Differ. 2016;23:197-206 pubmed publisher
Virts E, Jankowska A, MacKay C, Glaas M, Wiek C, Kelich S, et al. AluY-mediated germline deletion, duplication and somatic stem cell reversion in UBE2T defines a new subtype of Fanconi anemia. Hum Mol Genet. 2015;24:5093-108 pubmed publisher
Kinoshita E, van Rossum Fikkert S, Sanchez H, Kertokalio A, Wyman C. Human RAD50 makes a functional DNA-binding complex. Biochimie. 2015;113:47-53 pubmed publisher
Huang Y, Chou W, Hsiung C, Hu L, Chu H, Shen C. FGFR2 regulates Mre11 expression and double-strand break repair via the MEK-ERK-POU1F1 pathway in breast tumorigenesis. Hum Mol Genet. 2015;24:3506-17 pubmed publisher
Ohara M, Funyu Y, Ebara S, Sakamoto Y, Seki R, Iijima K, et al. Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination. J Radiat Res. 2014;55:690-8 pubmed publisher
Tahara M, Inoue T, Sato F, Miyakura Y, Horie H, Yasuda Y, et al. The use of Olaparib (AZD2281) potentiates SN-38 cytotoxicity in colon cancer cells by indirect inhibition of Rad51-mediated repair of DNA double-strand breaks. Mol Cancer Ther. 2014;13:1170-80 pubmed publisher
Kong X, Ball A, Pham H, Zeng W, Chen H, Schmiesing J, et al. Distinct functions of human cohesin-SA1 and cohesin-SA2 in double-strand break repair. Mol Cell Biol. 2014;34:685-98 pubmed publisher
Floyd S, Pacold M, Huang Q, Clarke S, Lam F, Cannell I, et al. The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. Nature. 2013;498:246-50 pubmed publisher
Lee J, Mand M, Deshpande R, Kinoshita E, Yang S, Wyman C, et al. Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex. J Biol Chem. 2013;288:12840-51 pubmed publisher
Böhringer M, Obermeier K, Griner N, Waldraff D, Dickinson E, Eirich K, et al. siRNA screening identifies differences in the Fanconi anemia pathway in BALB/c-Trp53+/- with susceptibility versus C57BL/6-Trp53+/- mice with resistance to mammary tumors. Oncogene. 2013;32:5458-70 pubmed publisher
Simões M, Martins C, Ferreira F. Host DNA damage response facilitates African swine fever virus infection. Vet Microbiol. 2013;165:140-7 pubmed publisher
Zhu J, Zhou L, Wu G, Konig H, Lin X, Li G, et al. A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia. EMBO Mol Med. 2013;5:353-65 pubmed publisher
Ogiwara H, Kohno T. CBP and p300 histone acetyltransferases contribute to homologous recombination by transcriptionally activating the BRCA1 and RAD51 genes. PLoS ONE. 2012;7:e52810 pubmed publisher
Knies K, Schuster B, Ameziane N, Rooimans M, Bettecken T, de Winter J, et al. Genotyping of fanconi anemia patients by whole exome sequencing: advantages and challenges. PLoS ONE. 2012;7:e52648 pubmed publisher
Oplustilova L, Wolanin K, Mistrik M, Korinkova G, Simkova D, Bouchal J, et al. Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment. Cell Cycle. 2012;11:3837-50 pubmed publisher
Masuda Y, Noguchi K, Segawa H, Tanaka N, Katayama K, Mitsuhashi J, et al. Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin. Biochem Biophys Res Commun. 2011;415:305-12 pubmed publisher
Cheng Q, Barboule N, Frit P, Gomez D, Bombarde O, Couderc B, et al. Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res. 2011;39:9605-19 pubmed publisher
Baydoun H, Pancewicz J, Nicot C. Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair. Blood. 2011;117:5897-906 pubmed publisher
Stoepker C, Hain K, Schuster B, Hilhorst Hofstee Y, Rooimans M, Steltenpool J, et al. SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype. Nat Genet. 2011;43:138-41 pubmed publisher
Matsumoto Y, Miyamoto T, Sakamoto H, Izumi H, Nakazawa Y, Ogi T, et al. Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly. DNA Repair (Amst). 2011;10:314-21 pubmed publisher
Luo Y, Chen A, Qiu J. Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death. J Virol. 2011;85:133-45 pubmed publisher
Kuroda S, Fujiwara T, Shirakawa Y, Yamasaki Y, Yano S, Uno F, et al. Telomerase-dependent oncolytic adenovirus sensitizes human cancer cells to ionizing radiation via inhibition of DNA repair machinery. Cancer Res. 2010;70:9339-48 pubmed publisher
Momcilovic O, Knobloch L, Fornsaglio J, Varum S, Easley C, Schatten G. DNA damage responses in human induced pluripotent stem cells and embryonic stem cells. PLoS ONE. 2010;5:e13410 pubmed publisher
Machida K, McNamara G, Cheng K, Huang J, Wang C, Comai L, et al. Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes. J Immunol. 2010;185:6985-98 pubmed publisher
Balasubramanian N, Bai P, Buchek G, Korza G, Weller S. Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex. J Virol. 2010;84:12504-14 pubmed publisher
Gomez Godinez V, Wu T, Sherman A, Lee C, Liaw L, Zhongsheng Y, et al. Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation. Nucleic Acids Res. 2010;38:e202 pubmed publisher
Palagyi A, Neveling K, Plinninger U, Ziesch A, Targosz B, Denk G, et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer. 2010;9:127 pubmed publisher
Cha H, Lowe J, Li H, Lee J, Belova G, Bulavin D, et al. Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response. Cancer Res. 2010;70:4112-22 pubmed publisher
Chailleux C, Tyteca S, Papin C, Boudsocq F, Puget N, Courilleau C, et al. Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex. Biochem J. 2010;426:365-71 pubmed publisher
Lee J, Goodarzi A, Jeggo P, Paull T. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J. 2010;29:574-85 pubmed publisher
Moody C, Laimins L. Human papillomaviruses activate the ATM DNA damage pathway for viral genome amplification upon differentiation. PLoS Pathog. 2009;5:e1000605 pubmed publisher
Collaco R, Bevington J, Bhrigu V, Kalman Maltese V, Trempe J. Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways. Virology. 2009;392:24-33 pubmed publisher
Waltes R, Kalb R, Gatei M, Kijas A, Stumm M, Sobeck A, et al. Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder. Am J Hum Genet. 2009;84:605-16 pubmed publisher
Bartkova J, Tommiska J, Oplustilova L, Aaltonen K, Tamminen A, Heikkinen T, et al. Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene. Mol Oncol. 2008;2:296-316 pubmed publisher
Schwartz R, Carson C, Schuberth C, Weitzman M. Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase. J Virol. 2009;83:6269-78 pubmed publisher
Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori A, Gerrits B, et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res. 2009;37:2645-57 pubmed publisher
Carson C, Orazio N, Lee D, Suh J, Bekker Jensen S, Araujo F, et al. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection. EMBO J. 2009;28:652-62 pubmed publisher
Schwartz R, Lakdawala S, Eshleman H, Russell M, Carson C, Weitzman M. Distinct requirements of adenovirus E1b55K protein for degradation of cellular substrates. J Virol. 2008;82:9043-55 pubmed publisher
Lakdawala S, Schwartz R, Ferenchak K, Carson C, McSharry B, Wilkinson G, et al. Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation. J Virol. 2008;82:8362-72 pubmed publisher
Cervelli T, Palacios J, Zentilin L, Mano M, Schwartz R, Weitzman M, et al. Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins. J Cell Sci. 2008;121:349-57 pubmed publisher
Gregory D, Bachenheimer S. Characterization of mre11 loss following HSV-1 infection. Virology. 2008;373:124-36 pubmed publisher
Liu L, Bailey S, Okuka M, Muñoz P, Li C, Zhou L, et al. Telomere lengthening early in development. Nat Cell Biol. 2007;9:1436-41 pubmed
Schwartz R, Palacios J, Cassell G, Adam S, Giacca M, Weitzman M. The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. J Virol. 2007;81:12936-45 pubmed
Zhong Z, Jiang W, Cesare A, Neumann A, Wadhwa R, Reddel R. Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres. J Biol Chem. 2007;282:29314-22 pubmed
Bentle M, Reinicke K, Dong Y, Bey E, Boothman D. Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone. Cancer Res. 2007;67:6936-45 pubmed
Potts P, Yu H. The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins. Nat Struct Mol Biol. 2007;14:581-90 pubmed
Cho Y, Hong T, Hong S, Guo H, Yu H, Kim D, et al. PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J Biol Chem. 2007;282:20395-406 pubmed
Bae N, Baumann P. A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends. Mol Cell. 2007;26:323-34 pubmed
Lin X, Liu C, Gao Q, Zhang X, Wu G, Lee W. RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival. Mol Cell Biol. 2007;27:4905-16 pubmed
You K, Li L, Kim N, Kang H, Koh K, Chwae Y, et al. Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. PLoS Biol. 2007;5:e109 pubmed
Fleisig H, Orazio N, Liang H, Tyler A, Adams H, Weitzman M, et al. Adenoviral E1B55K oncoprotein sequesters candidate leukemia suppressor sequence-specific single-stranded DNA-binding protein 2 into aggresomes. Oncogene. 2007;26:4797-805 pubmed
Dote H, Burgan W, Camphausen K, Tofilon P. Inhibition of hsp90 compromises the DNA damage response to radiation. Cancer Res. 2006;66:9211-20 pubmed
Takemura H, Rao V, Sordet O, Furuta T, Miao Z, Meng L, et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem. 2006;281:30814-23 pubmed
Kong L, Meloni A, Nevins J. The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1. Mol Cell. 2006;22:63-71 pubmed
Myers J, Cortez D. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J Biol Chem. 2006;281:9346-50 pubmed
Tommiska J, Seal S, Renwick A, Barfoot R, Baskcomb L, Jayatilake H, et al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer. 2006;118:2911-6 pubmed
van der Burg M, van Veelen L, Verkaik N, Wiegant W, Hartwig N, Barendregt B, et al. A new type of radiosensitive T-B-NK+ severe combined immunodeficiency caused by a LIG4 mutation. J Clin Invest. 2006;116:137-45 pubmed
Mongiat Artus P, Miquel C, van der Aa M, Buhard O, Hamelin R, Soliman H, et al. Microsatellite instability and mutation analysis of candidate genes in urothelial cell carcinomas of upper urinary tract. Oncogene. 2006;25:2113-8 pubmed
Liu Y, Shevchenko A, Shevchenko A, Berk A. Adenovirus exploits the cellular aggresome response to accelerate inactivation of the MRN complex. J Virol. 2005;79:14004-16 pubmed
Kim J, Krasieva T, Kurumizaka H, Chen D, Taylor A, Yokomori K. Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells. J Cell Biol. 2005;170:341-7 pubmed
Koh K, Kang H, Li L, Kim N, You K, Yang E, et al. Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas. Lab Invest. 2005;85:1130-8 pubmed
van Veelen L, Cervelli T, van de Rakt M, Theil A, Essers J, Kanaar R. Analysis of ionizing radiation-induced foci of DNA damage repair proteins. Mutat Res. 2005;574:22-33 pubmed
Lilley C, Carson C, Muotri A, Gage F, Weitzman M. DNA repair proteins affect the lifecycle of herpes simplex virus 1. Proc Natl Acad Sci U S A. 2005;102:5844-9 pubmed
Fasching C, Bower K, Reddel R. Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies. Cancer Res. 2005;65:2722-9 pubmed
Angele S, Jones C, Reis Filho J, Fulford L, Treilleux I, Lakhani S, et al. Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast. J Clin Pathol. 2004;57:1179-84 pubmed
O Connor M, Safari A, Liu D, Qin J, Songyang Z. The human Rap1 protein complex and modulation of telomere length. J Biol Chem. 2004;279:28585-91 pubmed
Wilkinson D, Weller S. Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage respon. J Virol. 2004;78:4783-96 pubmed
Dork T, Bendix Waltes R, Wegner R, Stumm M. Slow progression of ataxia-telangiectasia with double missense and in frame splice mutations. Am J Med Genet A. 2004;126A:272-7 pubmed
Giannini G, Rinaldi C, Ristori E, Ambrosini M, Cerignoli F, Viel A, et al. Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability. Oncogene. 2004;23:2640-7 pubmed
Carson C, Schwartz R, Stracker T, Lilley C, Lee D, Weitzman M. The Mre11 complex is required for ATM activation and the G2/M checkpoint. EMBO J. 2003;22:6610-20 pubmed
Uziel T, Lerenthal Y, Moyal L, Andegeko Y, Mittelman L, Shiloh Y. Requirement of the MRN complex for ATM activation by DNA damage. EMBO J. 2003;22:5612-21 pubmed
Xu X, Stern D. NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors. FASEB J. 2003;17:1842-8 pubmed
Angele S, Treilleux I, Bremond A, Taniere P, Hall J. Altered expression of DNA double-strand break detection and repair proteins in breast carcinomas. Histopathology. 2003;43:347-53 pubmed
Stewart G, Wang B, Bignell C, Taylor A, Elledge S. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature. 2003;421:961-6 pubmed
Anderson L, Perkins N. Regulation of RelA (p65) function by the large subunit of replication factor C. Mol Cell Biol. 2003;23:721-32 pubmed
Hassane D, Lee R, Pickett C. Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells. Infect Immun. 2003;71:541-5 pubmed
Richie C, Peterson C, Lu T, Hittelman W, Carpenter P, Legerski R. hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage. Mol Cell Biol. 2002;22:8635-47 pubmed
Pichierri P, Averbeck D, Rosselli F. DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein. Hum Mol Genet. 2002;11:2531-46 pubmed
Kim J, Krasieva T, LaMorte V, Taylor A, Yokomori K. Specific recruitment of human cohesin to laser-induced DNA damage. J Biol Chem. 2002;277:45149-53 pubmed
Stracker T, Carson C, Weitzman M. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature. 2002;418:348-52 pubmed
Franchitto A, Pichierri P. Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest. J Cell Biol. 2002;157:19-30 pubmed
Harfst E, Cooper S, Neubauer S, Distel L, Grawunder U. Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome. Mol Immunol. 2000;37:915-29 pubmed
Tauchi H, Kobayashi J, Morishima K, Matsuura S, Nakamura A, Shiraishi T, et al. The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50[middle dot]hMRE11[middle dot]NBS1 complex DNA repair activity. J Biol Chem. 2001;276:12-5 pubmed
Paull T, Rogakou E, Yamazaki V, Kirchgessner C, Gellert M, Bonner W. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol. 2000;10:886-95 pubmed
Wang Y, Cortez D, Yazdi P, Neff N, Elledge S, Qin J. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 2000;14:927-39 pubmed
product information
Catalog No :
GTX70228
Product Name :
Rad50 antibody [13B3]
Host Species :
Mouse
Clonality :
Monoclonal
Product Type :
Primary antibody
Size :
100.00μg
List Price :
299 USD
list of Pubmed id :
24324008,14511253,19270065,20974981,12228239,12607005,15054841,15914204,12509469,20923785,21240277,16905549,15805271,17470549,15078960,16288216,11062235,15100233,10783165,21427292,21227757,18562516,17898048,16260778,14519663,12354779,21880593,19339345,17500065,22037577,19197236,18216333,12496208,19628243,19409520,20943970,19383352,20509860,21047968,16385572,17456004,18177684,20010693,22983061,17638905,16600870,16061690,11282395,12446782,14532133,20070254,10959836,15509680,21045143,17499040,21239475,17693401,16982765,16025146,11916980,16254336,15824307,20460517,14657032,12124628,15048091,16431910,16357942,17311003,17589526,17982445,23728299,23285190,23285130,23398667,23525106,23341130,18614635,19798429,20976220,24577941,24614819,25788520,25857663,25828805
Clone :
13B3
Isotype :
IgG1
Product Description :
Mouse monoclonal [13B3] to Rad50
CrossReactivity :
Hu, Ms, Mk, Rat
Background :
RAD50 is an essential gene for all cell types and the expression does not vary during cell cycle. It is an excellent loading control at the high molecular weight position (150KD), complementing GAPDH, beta-Actin and alpha-Tubulin at the lower molecular weight positions.
Immunogen :
Amino acids 1-425 of Rad50 expressed in E. coli.
Buffer :
Phosphate-buffered saline, pH 7.4, containing no preservatives
Uses :
ICC/IF, IHC, IP, WB
Concentration :
1mg/ml
General References :
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
more info or order :
GeneTex product webpage
company information
GeneTex
2456 Alton Pkwy
Irvine, CA92606
info@genetex.com
http://www.genetex.com
1-877-436-3839
headquarters: USA