Nuclear Matrix Protein p84 antibody [5E10] | GeneTex GTX70220 product information

product summary

company name :

GeneTex

product type :

antibody

product name :

Nuclear Matrix Protein p84 antibody [5E10]

catalog :

GTX70220

quantity :

100.00μg

price :

299 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5E10

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

GeneTex product webpage

citations: 59

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 3s1	Sifuentes Dominguez L, Li H, Llano E, Liu Z, Singla A, Patel A, et al. SCGN deficiency results in colitis susceptibility. elife. 2019;8: pubmed publisher
western blot; mouse; loading ...; fig s1a	Koronowski K, Kinouchi K, Welz P, Smith J, Zinna V, Shi J, et al. Defining the Independence of the Liver Circadian Clock. Cell. 2019;177:1448-1462.e14 pubmed publisher
western blot; human; loading ...; fig 1a	Taura M, Song E, Ho Y, Iwasaki A. Apobec3A maintains HIV-1 latency through recruitment of epigenetic silencing machinery to the long terminal repeat. Proc Natl Acad Sci U S A. 2019;116:2282-2289 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig s10	Wu M, Chen W, Lu Y, Zhu G, Hao L, Li Y. Gα13 negatively controls osteoclastogenesis through inhibition of the Akt-GSK3β-NFATc1 signalling pathway. Nat Commun. 2017;8:13700 pubmed publisher
western blot; mouse; fig 3	Kong S, Yang Y, Xu Y, Wang Y, Zhang Y, Melo Cardenas J, et al. Endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 controls B-cell immunity through degradation of the death receptor CD95/Fas. Proc Natl Acad Sci U S A. 2016;113:10394-9 pubmed publisher
western blot; human; 1:3500; loading ...; fig s9c	Marceau C, Puschnik A, Majzoub K, Ooi Y, Brewer S, Fuchs G, et al. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature. 2016;535:159-63 pubmed
western blot; human; fig 4	Starokadomskyy P, Gemelli T, Rios J, Xing C, Wang R, Li H, et al. DNA polymerase-? regulates the activation of type I interferons through cytosolic RNA:DNA synthesis. Nat Immunol. 2016;17:495-504 pubmed publisher
western blot; mouse; loading ...; fig 7b	Wang S, Hsu J, Ko C, Chiu N, Kan W, Lai M, et al. Astrocytic CCAAT/Enhancer-Binding Protein Delta Contributes to Glial Scar Formation and Impairs Functional Recovery After Spinal Cord Injury. Mol Neurobiol. 2016;53:5912-5927 pubmed publisher
western blot; human; loading ...; fig 4a	Goedeke L, Rotllan N, CanfrÃ¡n Duque A, Aranda J, RamÃrez C, Araldi E, et al. MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels. Nat Med. 2015;21:1280-9 pubmed publisher
western blot; human; 1:1000; fig 2	Damiani E, Puebla Osorio N, Gorbea E, Ullrich S. Platelet-Activating Factor Induces Epigenetic Modifications in Human Mast Cells. J Invest Dermatol. 2015;135:3034-3040 pubmed publisher
western blot; mouse; fig s2	Lin A, Beasley F, Olson J, Keller N, Shalwitz R, Hannan T, et al. Role of Hypoxia Inducible Factor-1α (HIF-1α) in Innate Defense against Uropathogenic Escherichia coli Infection. PLoS Pathog. 2015;11:e1004818 pubmed publisher
western blot; human; fig s5	Aguilar Arnal L, Katada S, Orozco Solis R, Sassone Corsi P. NAD(+)-SIRT1 control of H3K4 trimethylation through circadian deacetylation of MLL1. Nat Struct Mol Biol. 2015;22:312-8 pubmed publisher
western blot; mouse; 1:5000; fig 7	Tontonoz P, Cortez Toledo O, Wroblewski K, Hong C, Lim L, Carranza R, et al. The orphan nuclear receptor Nur77 is a determinant of myofiber size and muscle mass in mice. Mol Cell Biol. 2015;35:1125-38 pubmed publisher
western blot; human; fig 4	Lecona E, Narendra V, Reinberg D. USP7 cooperates with SCML2 to regulate the activity of PRC1. Mol Cell Biol. 2015;35:1157-68 pubmed publisher
western blot; human	Wang J, Xu X, Alontaga A, Chen Y, Liu Y. Impaired p32 regulation caused by the lymphoma-prone RECQ4 mutation drives mitochondrial dysfunction. Cell Rep. 2014;7:848-58 pubmed publisher
immunocytochemistry; mouse	Nini L, Zhang J, Pandey M, Panicker L, Simonds W. Expression of the G?5/R7-RGS protein complex in pituitary and pancreatic islet cells. Endocrine. 2012;42:214-7 pubmed publisher
	Li M, Xu X, Chang C, Zheng L, Shen B, Liu Y. SUMO2 conjugation of PCNA facilitates chromatin remodeling to resolve transcription-replication conflicts. Nat Commun. 2018;9:2706 pubmed publisher
	Gendarme M, Baumann J, Ignashkova T, Lindemann R, Reiling J. Image-based drug screen identifies HDAC inhibitors as novel Golgi disruptors synergizing with JQ1. Mol Biol Cell. 2017;28:3756-3772 pubmed publisher
	Damiani E, Puebla Osorio N, Lege B, Liu J, Neelapu S, Ullrich S. Platelet activating factor-induced expression of p21 is correlated with histone acetylation. Sci Rep. 2017;7:41959 pubmed publisher
	Ho S, Lee Y, Lin W. Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain. J Biol Chem. 2015;290:23026-38 pubmed publisher
	Zhu J, Chen H, Guo X, Qiu X, Hu C, Chamberlin A, et al. Synthesis, molecular modeling, and biological evaluation of novel RAD51 inhibitors. Eur J Med Chem. 2015;96:196-208 pubmed publisher
	Li M, Pokharel S, Wang J, Xu X, Liu Y. RECQ5-dependent SUMOylation of DNA topoisomerase I prevents transcription-associated genome instability. Nat Commun. 2015;6:6720 pubmed publisher
	Ip J, Ko J, Yu V, Chan K, Lam A, Law S, et al. A versatile orthotopic nude mouse model for study of esophageal squamous cell carcinoma. Biomed Res Int. 2015;2015:910715 pubmed publisher
	Patel J, Puck J, Srinivasan R, Brown C, Sunderam U, Kundu K, et al. Nijmegen breakage syndrome detected by newborn screening for T cell receptor excision circles (TRECs). J Clin Immunol. 2015;35:227-33 pubmed publisher
	Chen Y, Chiang H, Litchfield P, Pena M, Juang C, Riley D. Expression of Nek1 during kidney development and cyst formation in multiple nephron segments in the Nek1-deficient kat2J mouse model of polycystic kidney disease. J Biomed Sci. 2014;21:63 pubmed publisher
	Sweet R, Zastre J. HIF1-?-mediated gene expression induced by vitamin B1 deficiency. Int J Vitam Nutr Res. 2013;83:188-97 pubmed publisher
	Haga I, Pechenick Jowers T, Griffiths S, Haas J, Beard P. TRAF2 facilitates vaccinia virus replication by promoting rapid virus entry. J Virol. 2014;88:3664-77 pubmed publisher
	Eckel Mahan K, Patel V, de Mateo S, Orozco Solis R, Ceglia N, Sahar S, et al. Reprogramming of the circadian clock by nutritional challenge. Cell. 2013;155:1464-78 pubmed publisher
	Reiling J, Olive A, Sanyal S, Carette J, Brummelkamp T, Ploegh H, et al. A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens. Nat Cell Biol. 2013;15:1473-85 pubmed publisher
	Thomas R, Roberts D, Kubli D, Lee Y, Quinsay M, Owens J, et al. Loss of MCL-1 leads to impaired autophagy and rapid development of heart failure. Genes Dev. 2013;27:1365-77 pubmed publisher
	Sharma M, Salisbury R, Maurer E, Hussain S, Sulentic C. Gold nanoparticles induce transcriptional activity of NF-κB in a B-lymphocyte cell line. Nanoscale. 2013;5:3747-56 pubmed publisher
	Zhu J, Zhou L, Wu G, Konig H, Lin X, Li G, et al. A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia. EMBO Mol Med. 2013;5:353-65 pubmed publisher
	Wei P, Hsieh Y, Su M, Jiang X, Hsu P, Lo W, et al. Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease. Mol Cell. 2012;48:747-59 pubmed publisher
	Diamant G, Amir Zilberstein L, Yamaguchi Y, Handa H, Dikstein R. DSIF restricts NF-?B signaling by coordinating elongation with mRNA processing of negative feedback genes. Cell Rep. 2012;2:722-31 pubmed publisher
	Ramachandran S, Tran D, Klebba Faerber S, Kardinal C, Whetton A, Tamura T. An ataxia-telangiectasia-mutated (ATM) kinase mediated response to DNA damage down-regulates the mRNA-binding potential of THOC5. RNA. 2011;17:1957-66 pubmed publisher
	Pang T, Clarke M, Ghasem Zadeh A, Lee N, Davey R, MacLean H. A physiological role for androgen actions in the absence of androgen receptor DNA binding activity. Mol Cell Endocrinol. 2012;348:189-97 pubmed publisher
	Kanungo J, Chandrasekharappa S. Menin induces endodermal differentiation in aggregated P19 stem cells by modulating the retinoic acid receptors. Mol Cell Biochem. 2012;359:95-104 pubmed publisher
	Lee S, Chung J, Park M, Joo H, Lee E, Cho E, et al. Apurinic/apyrimidinic endonuclease 1 inhibits protein kinase C-mediated p66shc phosphorylation and vasoconstriction. Cardiovasc Res. 2011;91:502-9 pubmed publisher
	Chen Y, Chen C, Riley D, Chen P. Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR. Cell Cycle. 2011;10:655-63 pubmed
	Wang Y, Ozawa A, Zaman S, Prasad N, Chandrasekharappa S, Agarwal S, et al. The tumor suppressor protein menin inhibits AKT activation by regulating its cellular localization. Cancer Res. 2011;71:371-82 pubmed publisher
	Mancini A, Niemann Seyde S, Pankow R, El Bounkari O, Klebba Färber S, Koch A, et al. THOC5/FMIP, an mRNA export TREX complex protein, is essential for hematopoietic primitive cell survival in vivo. BMC Biol. 2010;8:1 pubmed publisher
	Wang X, Chinnam M, Wang J, Wang Y, Zhang X, Marcon E, et al. Thoc1 deficiency compromises gene expression necessary for normal testis development in the mouse. Mol Cell Biol. 2009;29:2794-803 pubmed publisher
	El Bounkari O, Guria A, Klebba Faerber S, Claussen M, Pieler T, Griffiths J, et al. Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP). FEBS Lett. 2009;583:13-8 pubmed publisher
	Yang J, Li Y, Khoury T, Alrawi S, Goodrich D, Tan D. Relationships of hHpr1/p84/Thoc1 expression to clinicopathologic characteristics and prognosis in non-small cell lung cancer. Ann Clin Lab Sci. 2008;38:105-12 pubmed
	Byun H, Song J, Kim Y, Piao L, Won M, Park K, et al. Caspase-8 has an essential role in resveratrol-induced apoptosis of rheumatoid fibroblast-like synoviocytes. Rheumatology (Oxford). 2008;47:301-8 pubmed publisher
	Bhandari R, Juluri K, Resnick A, Snyder S. Gene deletion of inositol hexakisphosphate kinase 1 reveals inositol pyrophosphate regulation of insulin secretion, growth, and spermiogenesis. Proc Natl Acad Sci U S A. 2008;105:2349-53 pubmed publisher
	Ji Y, Prasad N, Novotny E, Kaur S, Elkahloun A, Chen Y, et al. Mouse embryo fibroblasts lacking the tumor suppressor menin show altered expression of extracellular matrix protein genes. Mol Cancer Res. 2007;5:1041-51 pubmed
	Nini L, Waheed A, Panicker L, Czapiga M, Zhang J, Simonds W. R7-binding protein targets the G protein beta 5/R7-regulator of G protein signaling complex to lipid rafts in neuronal cells and brain. BMC Biochem. 2007;8:18 pubmed
	Li Y, Lin A, Zhang X, Wang Y, Wang X, Goodrich D. Cancer cells and normal cells differ in their requirements for Thoc1. Cancer Res. 2007;67:6657-64 pubmed
	Birle D, Hedley D. Suppression of the hypoxia-inducible factor-1 response in cervical carcinoma xenografts by proteasome inhibitors. Cancer Res. 2007;67:1735-43 pubmed
	Mancini A, El Bounkari O, Norrenbrock A, Scherr M, Schaefer D, Eder M, et al. FMIP controls the adipocyte lineage commitment of C2C12 cells by downmodulation of C/EBP alpha. Oncogene. 2007;26:1020-7 pubmed
	Wang X, Chang Y, Li Y, Zhang X, Goodrich D. Thoc1/Hpr1/p84 is essential for early embryonic development in the mouse. Mol Cell Biol. 2006;26:4362-7 pubmed
	Li Y, Wang X, Zhang X, Goodrich D. Human hHpr1/p84/Thoc1 regulates transcriptional elongation and physically links RNA polymerase II and RNA processing factors. Mol Cell Biol. 2005;25:4023-33 pubmed
	Gasparri F, Sola F, Locatelli G, Muzio M. The death domain protein p84N5, but not the short isoform p84N5s, is cell cycle-regulated and shuttles between the nucleus and the cytoplasm. FEBS Lett. 2004;574:13-9 pubmed
	Agarwal S, Novotny E, Crabtree J, Weitzman J, Yaniv M, Burns A, et al. Transcription factor JunD, deprived of menin, switches from growth suppressor to growth promoter. Proc Natl Acad Sci U S A. 2003;100:10770-5 pubmed
	Jaubert J, Cheng J, Segre J. Ectopic expression of kruppel like factor 4 (Klf4) accelerates formation of the epidermal permeability barrier. Development. 2003;130:2767-77 pubmed
	Sukhodolets K, Hickman A, Agarwal S, Sukhodolets M, Obungu V, Novotny E, et al. The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene. Mol Cell Biol. 2003;23:493-509 pubmed
	Sunn K, Cock T, Crofts L, Eisman J, Gardiner E. Novel N-terminal variant of human VDR. Mol Endocrinol. 2001;15:1599-609 pubmed
	Bounpheng M, Melnikova I, Dodds S, Chen H, Copeland N, Gilbert D, et al. Characterization of the mouse JAB1 cDNA and protein. Gene. 2000;242:41-50 pubmed

product information

Catalog No :

GTX70220

Product Name :

Nuclear Matrix Protein p84 antibody [5E10]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

List Price :

299 USD

list of Pubmed id :

24429366,24360271,24185178,18469354,23041311,20051105,18276737,21833538,22322946,19307311,21301226,21937706,21872641,19059247,15358532,17308115,21467074,15870275,17880698,12736219,17638875,12960363,21127195,23123197,12509449,17951404,16705185,18268345,10721695,11518809,16909111,23503581,23341130,23788623,24746816,24846908,25030234,25751424, 25677497,25834829,25751424,25874343,25851487

Clone :

5E10

Isotype :

IgG2b

Product Description :

Mouse monoclonal [5E10] to Nuclear Matrix Protein p84

CrossReactivity :

Hu, Ms, Mk, Rat

Background :

P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Immunogen :

Amino acids 15-374 of human p84 expressed in E. coli.

Buffer :

Phosphate-buffered saline, pH 7.4, containing no preservatives

Uses :

ICC/IF, IHC, IP, WB, ChIP assay

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Image Legend_4 :

Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE GTX70220 diluted at 1:1000

Image Legend_5 :

Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7.5% SDS PAGE GTX70220 diluted at 1:1000

Image Legend_6 :

Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7.5% SDS PAGE GTX70220 diluted at 1:1000

Image Legend_7 :

p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] (GTX70220) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (GTX70220) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Image Legend_8 :

p84 antibody [5E10] detects p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p84 protein stained by p84 antibody [5E10] (GTX70220) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

more info or order :

GeneTex product webpage