FEN1 antibody [4E7] | GeneTex GTX70185 product information

product summary

company name :

GeneTex

product type :

antibody

product name :

FEN1 antibody [4E7]

catalog :

GTX70185

quantity :

100.00μg

price :

299 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4E7

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

GeneTex product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 1b western blot; human; loading ...; fig 2a	He L, Luo L, Zhu H, Yang H, Zhang Y, Wu H, et al. FEN1 promotes tumor progression and confers cisplatin resistance in non-small-cell lung cancer. Mol Oncol. 2017;11:640-654 pubmed publisher
immunoprecipitation; human; loading ...; fig 5a western blot; human; loading ...; fig 5a	Liu S, Lu G, Ali S, Liu W, Zheng L, Dai H, et al. Okazaki fragment maturation involves Î±-segment error editing by the mammalian FEN1/MutSÎ± functional complex. EMBO J. 2015;34:1829-43 pubmed publisher
western blot; human	Liu P, Qian L, Sung J, de Souza Pinto N, Zheng L, Bogenhagen D, et al. Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria. Mol Cell Biol. 2008;28:4975-87 pubmed publisher
western blot; human	Guo Z, Qian L, Liu R, Dai H, Zhou M, Zheng L, et al. Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair. Mol Cell Biol. 2008;28:4310-9 pubmed publisher
western blot; human	Guo Z, Chavez V, Singh P, Finger L, Hang H, Hegde M, et al. Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways. J Mol Biol. 2008;377:679-90 pubmed publisher
	Kitamura K, Que L, Shimadu M, Koura M, Ishihara Y, Wakae K, et al. Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus. PLoS Pathog. 2018;14:e1007124 pubmed publisher
	Li Z, Liu B, Jin W, Wu X, Zhou M, Liu V, et al. hDNA2 nuclease/helicase promotes centromeric DNA replication and genome stability. EMBO J. 2018;37: pubmed publisher
	Tann A, Boldogh I, Meiss G, Qian W, Van Houten B, Mitra S, et al. Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair. J Biol Chem. 2011;286:31975-83 pubmed publisher
	Guo Z, Zheng L, Xu H, Dai H, Zhou M, Pascua M, et al. Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. Nat Chem Biol. 2010;6:766-73 pubmed publisher
	Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton J, et al. DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst). 2010;9:109-19 pubmed publisher
	Masaoka A, Horton J, Beard W, Wilson S. DNA polymerase beta and PARP activities in base excision repair in living cells. DNA Repair (Amst). 2009;8:1290-9 pubmed publisher
	Kong X, Mohanty S, Stephens J, Heale J, Gomez Godinez V, Shi L, et al. Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells. Nucleic Acids Res. 2009;37:e68 pubmed publisher
	Guo Z, Zheng L, Dai H, Zhou M, Xu H, Shen B. Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity. Nucleic Acids Res. 2009;37:3431-41 pubmed publisher
	Szczesny B, Tann A, Longley M, Copeland W, Mitra S. Long patch base excision repair in mammalian mitochondrial genomes. J Biol Chem. 2008;283:26349-56 pubmed publisher
	Prasad R, Liu Y, Deterding L, Poltoratsky V, Kedar P, Horton J, et al. HMGB1 is a cofactor in mammalian base excision repair. Mol Cell. 2007;27:829-41 pubmed
	Heale J, Ball A, Schmiesing J, Kim J, Kong X, Zhou S, et al. Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair. Mol Cell. 2006;21:837-48 pubmed
	Kedar P, Kim S, Robertson A, Hou E, Prasad R, Horton J, et al. Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen. J Biol Chem. 2002;277:31115-23 pubmed
	Shibata Y, Nakamura T. Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay. J Biol Chem. 2002;277:746-54 pubmed

product information

Catalog No :

GTX70185

Product Name :

FEN1 antibody [4E7]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

List Price :

299 USD

list of Pubmed id :

18541666,17803946,19336415,18291413,18635552,19357094,21768646,18443037,20006562,20729856,11687589,12063248,16543152,19748837

Clone :

4E7

Isotype :

IgG1

Product Description :

Mouse monoclonal [4E7] FEN1

CrossReactivity :

Hu, Ms

Background :

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Immunogen :

Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.

Buffer :

Phosphate-buffered saline, pH 7.4, containing no preservatives

Uses :

ICC/IF, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

more info or order :

GeneTex product webpage