Estrogen Receptor beta antibody [7B10.7] | GeneTex GTX70182 product information

product summary

company name :

GeneTex

product type :

antibody

product name :

Estrogen Receptor beta antibody [7B10.7]

catalog :

GTX70182

quantity :

100.00μg

price :

299 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7B10.7

reactivity :

human

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

more info or order :

GeneTex product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; loading ...; fig 1c	Nelson A, Groen A, Miller J, Warren A, Holmes K, Tarulli G, et al. Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity. Mol Cell Endocrinol. 2017;440:138-150 pubmed publisher
chromatin immunoprecipitation; human; fig 1	Charn T, Liu E, Chang E, Lee Y, Katzenellenbogen J, Katzenellenbogen B. Genome-wide dynamics of chromatin binding of estrogen receptors alpha and beta: mutual restriction and competitive site selection. Mol Endocrinol. 2010;24:47-59 pubmed publisher
chromatin immunoprecipitation; human	Vivar O, Saunier E, Leitman D, Firestone G, Bjeldanes L. Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane. Endocrinology. 2010;151:1662-7 pubmed publisher
	Chang E, Charn T, Park S, Helferich W, Komm B, Katzenellenbogen J, et al. Estrogen Receptors alpha and beta as determinants of gene expression: influence of ligand, dose, and chromatin binding. Mol Endocrinol. 2008;22:1032-43 pubmed publisher
	Jiang Y, Gong P, Madak-Erdogan Z, Martin T, Jeyakumar M, Carlson K, et al. Mechanisms enforcing the estrogen receptor β selectivity of botanical estrogens. FASEB J. 2013;27:4406-18 pubmed publisher
	Krishnamurthy N, Hu Y, Siedlak S, Doughman Y, Watanabe M, Montano M. Induction of quinone reductase by tamoxifen or DPN protects against mammary tumorigenesis. FASEB J. 2012;26:3993-4002 pubmed publisher
	Bianco N, Perry G, Smith M, Templeton D, Montano M. Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage. Mol Endocrinol. 2003;17:1344-55 pubmed
	Monsivais D, Dyson M, Yin P, Coon J, Navarro A, Feng G, et al. ERβ- and prostaglandin E2-regulated pathways integrate cell proliferation via Ras-like and estrogen-regulated growth inhibitor in endometriosis. Mol Endocrinol. 2014;28:1304-15 pubmed publisher
	Burakov D, Crofts L, Chang C, Freedman L. Reciprocal recruitment of DRIP/mediator and p160 coactivator complexes in vivo by estrogen receptor. J Biol Chem. 2002;277:14359-62 pubmed
	Cvoro A, Tzagarakis-Foster C, Tatomer D, Paruthiyil S, Fox M, Leitman D. Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression. Mol Cell. 2006;21:555-64 pubmed
	Madak-Erdogan Z, Charn T, Jiang Y, Liu E, Katzenellenbogen J, Katzenellenbogen B. Integrative genomics of gene and metabolic regulation by estrogen receptors α and β, and their coregulators. Mol Syst Biol. 2013;9:676 pubmed publisher
	Paruthiyil S, Cvoro A, Tagliaferri M, Cohen I, Shtivelman E, Leitman D. Estrogen receptor β causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2. Breast Cancer Res Treat. 2011;129:777-84 pubmed publisher
	Tee M, Rogatsky I, Tzagarakis-Foster C, Cvoro A, An J, Christy R, et al. Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta. Mol Biol Cell. 2004;15:1262-72 pubmed
	Cvoro A, Paruthiyil S, Jones J, Tzagarakis-Foster C, Clegg N, Tatomer D, et al. Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract. Endocrinology. 2007;148:538-47 pubmed
	Ruddy S, Lau R, Cabrita M, McGregor C, McKay B, Murphy L, et al. Preferential estrogen receptor β ligands reduce Bcl-2 expression in hormone-resistant breast cancer cells to increase autophagy. Mol Cancer Ther. 2014;13:1882-93 pubmed publisher
	Vivar O, Zhao X, Saunier E, Griffin C, Mayba O, Tagliaferri M, et al. Estrogen receptor beta binds to and regulates three distinct classes of target genes. J Biol Chem. 2010;285:22059-66 pubmed publisher

product information

Catalog No :

GTX70182

Product Name :

Estrogen Receptor beta antibody [7B10.7]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

List Price :

299 USD

list of Pubmed id :

20160136,14699072,21120602,16483936,20404318,19897598,22700872,17095596,12714703,11893728,18258689,23774759,23882126,24785256,24992181

Clone :

7B10.7

Isotype :

IgG1

Product Description :

Mouse monoclonal antibody to human estrogen receptor beta

CrossReactivity :

Background :

The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).

Immunogen :

Amino acids 1-153 of human ER-§ expressed in E. coli.

Buffer :

Phosphate-buffered saline, pH 7.4, containing no preservatives

Uses :

IP, WB, ChIP assay

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

more info or order :

GeneTex product webpage