DNA ligase III antibody [1F3] | GeneTex GTX70143 product information

product summary

company name :

GeneTex

product type :

antibody

product name :

DNA ligase III antibody [1F3]

catalog :

GTX70143

quantity :

100.00μg

price :

299 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1F3

reactivity :

human, mouse, chicken

application :

western blot, immunocytochemistry, neutralization, immunoprecipitation

more info or order :

GeneTex product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig s2	Masaoka A, Gassman N, Horton J, Kedar P, Witt K, Hobbs C, et al. Interaction between DNA Polymerase β and BRCA1. PLoS ONE. 2013;8:e66801 pubmed
western blot; human; fig 4	Ebert F, Weiss A, Bültemeyer M, Hamann I, Hartwig A, Schwerdtle T. Arsenicals affect base excision repair by several mechanisms. Mutat Res. 2011;715:32-41 pubmed publisher
	Paul K, Wang M, Mladenov E, Bencsik-Theilen A, Bednar T, Wu W, et al. DNA ligases I and III cooperate in alternative non-homologous end-joining in vertebrates. PLoS ONE. 2013;8:e59505 pubmed publisher
	Singh S, Wu W, Zhang L, Klammer H, Wang M, Iliakis G. Widespread dependence of backup NHEJ on growth state: ramifications for the use of DNA-PK inhibitors. Int J Radiat Oncol Biol Phys. 2011;79:540-8 pubmed publisher
	Arakawa H, Bednar T, Wang M, Paul K, Mladenov E, Bencsik-Theilen A, et al. Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells. Nucleic Acids Res. 2012;40:2599-610 pubmed publisher
	Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton J, et al. DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst). 2010;9:109-19 pubmed publisher
	Dong Z, Tomkinson A. ATM mediates oxidative stress-induced dephosphorylation of DNA ligase IIIalpha. Nucleic Acids Res. 2006;34:5721-279 pubmed
	Horton J, Stefanick D, Prasad R, Gassman N, Kedar P, Wilson S. Base excision repair defects invoke hypersensitivity to PARP inhibition. Mol Cancer Res. 2014;12:1128-39 pubmed publisher
	Okano S, Lan L, Tomkinson A, Yasui A. Translocation of XRCC1 and DNA ligase IIIalpha from centrosomes to chromosomes in response to DNA damage in mitotic human cells. Nucleic Acids Res. 2005;33:422-9 pubmed
	Horton J, Stefanick D, Gassman N, Williams J, Gabel S, Cuneo M, et al. Preventing oxidation of cellular XRCC1 affects PARP-mediated DNA damage responses. DNA Repair (Amst). 2013;12:774-85 pubmed publisher
	Moscariello M, Iliakis G. Effects of chromatin decondensation on alternative NHEJ. DNA Repair (Amst). 2013;12:972-81 pubmed publisher
	Guo Z, Zheng L, Dai H, Zhou M, Xu H, Shen B. Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity. Nucleic Acids Res. 2009;37:3431-41 pubmed publisher
	Prasad R, Williams J, Hou E, Wilson S. Pol β associated complex and base excision repair factors in mouse fibroblasts. Nucleic Acids Res. 2012;40:11571-82 pubmed publisher
	Windhofer F, Wu W, Iliakis G. Low levels of DNA ligases III and IV sufficient for effective NHEJ. J Cell Physiol. 2007;213:475-83 pubmed
	Wang H, Rosidi B, Perrault R, Wang M, Zhang L, Windhofer F, et al. DNA ligase III as a candidate component of backup pathways of nonhomologous end joining. Cancer Res. 2005;65:4020-30 pubmed
	Leppard J, Dong Z, Mackey Z, Tomkinson A. Physical and functional interaction between DNA ligase IIIalpha and poly(ADP-Ribose) polymerase 1 in DNA single-strand break repair. Mol Cell Biol. 2003;23:5919-27 pubmed

product information

Catalog No :

GTX70143

Product Name :

DNA ligase III antibody [1F3]

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

List Price :

299 USD

list of Pubmed id :

21782832,15653642,22127868,20006562,19336415,17492771,12897160,15899791,23042675,20950945,17040896,23555685,23826138,23871146,24051048,24770870

Clone :

1F3

Isotype :

IgG1

Product Description :

Mouse monoclonal [1F3] to DNA ligase III

CrossReactivity :

Hu, Ms, Chk

Background :

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa (§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.

Immunogen :

Recombinant full-length human DNA Ligase III-b.

Buffer :

Phosphate-buffered saline, pH 7.2, containing no preservative

Uses :

Blocking, ICC/IF, IP, WB

Concentration :

1mg/ml

General References :

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

more info or order :

GeneTex product webpage