BRCA1 antibody [6B4] - ChIP grade | GeneTex GTX70115 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

BRCA1 antibody [6B4] - ChIP grade

catalog :

GTX70115

quantity :

100.00μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6B4

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; fig 7	Morales J, Richard P, Patidar P, Motea E, Dang T, Manley J, et al. XRN2 Links Transcription Termination to DNA Damage and Replication Stress. PLoS Genet. 2016;12:e1006107 pubmed publisher
	Lajud S, Nagda D, Yamashita T, Zheng J, Tanaka N, Abuzeid W, et al. Dual disruption of DNA repair and telomere maintenance for the treatment of head and neck cancer. Clin Cancer Res. 2014;20:6465-78 pubmed publisher
	Bernard Gallon D, Dechelotte P, Le Corre L, Chalabi N, Vissac Sabatier C, Bignon Y. Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia. Anticancer Res. 2004;24:321-4 pubmed
	Li S, Ting N, Zheng L, Chen P, Ziv Y, Shiloh Y, et al. Functional link of BRCA1 and ataxia telangiectasia gene product in DNA damage response. Nature. 2000;406:210-5 pubmed

product information

Catalog No :

GTX70115

Product Name :

BRCA1 antibody [6B4] - ChIP grade

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

list of Pubmed id :

10910365,15015615,25324139

Clone :

6B4

Isotype :

IgG1

Product Description :

Mouse monoclonal [6B4] to BRCA1

CrossReactivity :

Hu, Ms

Background :

BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.

Immunogen :

BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

Specificity :

This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.

Buffer :

PBS

Uses :

ICC/IF, IHC, IP, WB, ChIP assay

Concentration :

2.75mg/ml

General References :

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Image Legend_5 :

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 microgram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

Image Legend_6 :

BRCA1 antibody 6B4 (GTX70115) was used for Western blot assay at 1:1000 antibody dilution. Western blot assay was performed using MCF7 (human) and MEF(mouse embryonic fibroblast) cell lysate separated in a 5-15% gradient gel. Observed molecular weight of BRCA1: 220 KD. Predicted molecular weight of BRCA1: 220 KD.

Image Legend_7 :

BRCA1 antibody detects BRCA1 protein by western blot analysis. Whole cell extracts (30 and 50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody (GTX70115) at a dilution of 1:500.