BRCA1 antibody [17F8] - ChIP grade | GeneTex GTX70111 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

GeneTex

product type :

antibody

product name :

BRCA1 antibody [17F8] - ChIP grade

catalog :

GTX70111

quantity :

100.00μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

17F8

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 6a	Urban V, Dobrovolna J, Huhn D, Fryzelkova J, Bartek J, Janscak P. RECQ5 helicase promotes resolution of conflicts between replication and transcription in human cells. J Cell Biol. 2016;214:401-15 pubmed publisher
	Buisson R, Masson J. PALB2 self-interaction controls homologous recombination. Nucleic Acids Res. 2012;40:10312-23 pubmed publisher
	Bosviel R, Dumollard E, Dechelotte P, Bignon Y, Bernard Gallon D. Can soy phytoestrogens decrease DNA methylation in BRCA1 and BRCA2 oncosuppressor genes in breast cancer?. OMICS. 2012;16:235-44 pubmed publisher
	Vardi A, Bosviel R, Rabiau N, Adjakly M, Satih S, Dechelotte P, et al. Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells. In Vivo. 2010;24:393-400 pubmed
	Bernard Gallon D, Dechelotte P, Le Corre L, Chalabi N, Vissac Sabatier C, Bignon Y. Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia. Anticancer Res. 2004;24:321-4 pubmed
	Ongusaha P, Ouchi T, Kim K, Nytko E, Kwak J, Duda R, et al. BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest. Oncogene. 2003;22:3749-58 pubmed
	Bernard Gallon D, Dechelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, et al. BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite. Breast Cancer Res. 2001;3:61-5 pubmed
	Lu M, Arrick B. Transactivation of the p21 promoter by BRCA1 splice variants in mammary epithelial cells: evidence for both common and distinct activities of wildtype and mutant forms. Oncogene. 2000;19:6351-60 pubmed
	de Toledo S, Azzam E, Dahlberg W, Gooding T, Little J. ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation. Oncogene. 2000;19:6185-93 pubmed
	Arizti P, Fang L, Park I, Yin Y, Solomon E, Ouchi T, et al. Tumor suppressor p53 is required to modulate BRCA1 expression. Mol Cell Biol. 2000;20:7450-9 pubmed
	Altiok S, Batt D, Altiok N, Papautsky A, Downward J, Roberts T, et al. Heregulin induces phosphorylation of BRCA1 through phosphatidylinositol 3-Kinase/AKT in breast cancer cells. J Biol Chem. 1999;274:32274-8 pubmed
	Bernard Gallon D, Dechelotte P, Rio P, Bignon Y. Expression of human BRCA1 and BRCA2 proteins in lung from a fetus at 19 weeks' gestation. Int J Cancer. 1999;82:771-3 pubmed
	Yoshikawa K, Honda K, Inamoto T, Shinohara H, Yamauchi A, Suga K, et al. Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases. Clin Cancer Res. 1999;5:1249-61 pubmed
	Bernard Gallon D, Dechelotte P, Rio P, Bignon Y. BRCA1 and BRCA2 proteins are expressed in milk fat globules. Int J Cancer. 1999;81:839-43 pubmed

product information

Catalog No :

GTX70111

Product Name :

BRCA1 antibody [17F8] - ChIP grade

Host Species :

Mouse

Clonality :

Monoclonal

Product Type :

Primary antibody

Size :

100.00μg

list of Pubmed id :

12802282,10542266,10417779,22941656,11003642,10328242,16260778,10389907,20668305,11175350,15015615,11175332,22339411,11250747,15208681

Clone :

17F8

Isotype :

IgG1

Product Description :

Mouse monoclonal [17F8] to BRCA1

CrossReactivity :

Hu, Ms

Background :

BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the Aexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.

Immunogen :

Protein fragment expressed in E. coli corresponding to amino acids 762-1315.

Specificity :

This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.

Buffer :

Phosphate-buffered saline, pH7.2 containing no preservatives

Uses :

ELISA, ICC/IF, IHC, IHC-P, IP, WB, ChIP assay

Concentration :

1.81mg/ml

General References :

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Image Legend_4 :

Sample (30 ug of whole cell lysate) A: 293T B: HeLa 5% SDS PAGE GTX70111 diluted at 1:500

Image Legend_5 :

BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500