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product summary
company name :
GeneTex
product type :
antibody
product name :
Histone H3K9K14ac (acetyl Lys9/Lys14) antibody - ChIP grade
catalog :
GTX60822
quantity :
50.00μg
price :
369 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
antigen modification :
acetylated
reactivity :
human, mouse
application :
western blot, ELISA, immunocytochemistry, ChIP-Seq
product information
Catalog No :
GTX60822
Product Name :
Histone H3K9K14ac (acetyl Lys9/Lys14) antibody - ChIP grade
Host Species :
Rabbit
Clonality :
Polyclonal
Antigen Modification :
Acetylated
Product Type :
Primary antibody
Size :
50.00μg
List Price :
369 USD
Product Description :
Rabbit polyclonal antibody to Histone H3K9K14ac (acetyl Lys9/Lys14)
CrossReactivity :
Hu, Ms
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Immunogen :
Polyclonal antibody raised in rabbit against the region of histone H3 containing the acetylated lysines 9 and 14 (H3K9/14ac), using a KLH-conjugated synthetic peptide.
Buffer :
PBS containing 0.05% azide and 0.05% ProClin 300.
Uses :
Dot, ELISA, ICC/IF, WB, ChIP assay, ChIP-seq
Concentration :
0.81mg/ml
General References :
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Image Legend_4 :
ICC/IF analysis of HeLa cells using H3K9acK14ac antibody (green) and DAPI (blue). Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with H3K9acK14ac antibody at a dilution of 1:200 in.
Image Legend_5 :
ChIP was performed with HeLa extracts and either control IgG (2 μg) or Histone H3K9acK14ac (acetyl Lys9/Lys14) antibody (1, 2, 5 and 10 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to positive controls the promoter of the active genes GAPDH and EIF4A2, and to negative controls the coding region of the inactive MB gene and the Sat2 satellite repeat.
company information
GeneTex
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