This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
GeneTex
product type :
antibody
product name :
Histone H3K27ac (acetyl Lys27) antibody - ChIP grade
catalog :
GTX60815
quantity :
50.00μg
price :
369 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
antigen modification :
acetylated
reactivity :
human, mouse
application :
western blot, ELISA, immunocytochemistry, ChIP-Seq
product information
Catalog No :
GTX60815
Product Name :
Histone H3K27ac (acetyl Lys27) antibody - ChIP grade
Host Species :
Rabbit
Clonality :
Polyclonal
Antigen Modification :
Acetylated
Product Type :
Primary antibody
Size :
50.00μg
List Price :
369 USD
Product Description :
Rabbit polyclonal antibody to H3 (acetyl Lys27)
CrossReactivity :
Hu, Ms
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Immunogen :
Polyclonal antibody raised in rabbit against the region of histone H3 containing the acetylated lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide.
Buffer :
PBS containing 0.05% azide and 0.05% ProClin 300.
Uses :
Dot, ELISA, ICC/IF, WB, ChIP assay, ChIP-seq
Concentration :
2.80mg/ml
General References :
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Image Legend_4 :
ChIP was performed with HeLa extracts and either control IgG (2 μg) or Histone H3K27ac (acetyl Lys27) antibody (1, 2, 5 and 10 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to positive controls ACTB and EIF4A2 promoters, and to negative controls MYT1 and TSH2B genes.
Image Legend_5 :
ChIP was performed with K562 extracts and either control IgG (1 μg) or Histone H3K27ac (acetyl Lys27) antibody (0.2, 0.5, 1 and 2 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to positive controls GAPDH and EIF4A2 promoters, and to negative controls MB and MYT1 genes.
Image Legend_6 :
ELISA was performed using a serial dilution of Histone H3K27ac (acetyl Lys27) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,300.
company information
GeneTex
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